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- Analysis of Petiole Sap Nutrients Using Rapid and Standard Methods and Its Relation to Leaf Analysis of Fertilized Malus domestica cv. GalaPublication . Mota, Mariana; Martins, M.; Sprey, Layanne; Maurício, Anabela; Rosa, Cristina; Faria, João; Martins, Miguel; Sousa, Miguel L. de; Santos, Ricardo; Sousa, Rui M. de; Ribeiro, Henrique; Oliveira, Cristina M.Currently, fertilization decisions in apple orchards are based on soil and leaf analyses while the leaf material is sampled after the growing season, usually in June–July (90–110 days after full bloom). This approach is inefficient, as the information becomes available later than the growing season and is therefore only useful in supporting fertilization decisions for the next year, not the current one. To establish a method that provides useful information for fertilization decisions earlier in the growth cycle, our research focused on the assessment of the nutrient content of petiole sap using different methods, the standard method and the rapid method using a reflectometer. For this study, in 2021, four ‘Gala’ orchards were fertilized with different N–P–K levels. Macro and micronutrients were determined in leaves and petiole sap at 45 and 90–110 days after full bloom (DAFB) using standard laboratory methods and the reflectometer. When leaf analysis at 45 and 90–110 DAFB was compared with petiole sap analysis at the same time point, no significant correlations were found between the nutrient contents in leaf material and petiole sap, with the exception of calcium. However, positive results were obtained regarding the correlation between reflectometer determination and standard laboratory analyses. The regression analysis revealed high determination coefficients for N-NO3 − (R2 = 0.703), K+ (R2 = 0.705), Ca2+ (R2 = 0.715), and Mg2+ (R2 = 0.780) between standard laboratory methods and the reflectometer. These results suggest that the reflectometer enables a real-time diagnostic tool for monitoring nutrient status throughout the growth cycle, particularly key nutrients related to fruit quality. The N–P–K fertilization strategies had no influence on the nutrient content of leaves or petiole sap. The nutrient content of both sample types varied depending on the orchard.
- Rapid Sap Nutrient Analysis Methods in Malus Domestica Borkh Cv. ‘Gala’Publication . Almeida, Patrícia da Costa; Oliveira, Cristina M.; Mota, Mariana; Ribeiro, HenriqueSap quick tests are well established in vegetable crops. However, there is less equivalent investigation for perennial crops, such as apple trees. Accessing the nutrient content, as opposed to the foliar analysis, would increase the opportunity of adjusting the fertilization, along the growing cycle. This work evaluates the relation between the NO3 −, K+, Ca2+, Mg2+, PO4 3- and NH4 + contents in apple petiole sap, measured with the RQflex® test strips and laboratory methods to assess the possibility of using this tool with accuracy in in-situ sap analysis. Petiole samples were collected from six apple tree orchards, frozen and pressed. Dilution was mandatory for all nutrient determination, except NO3 −. The dilution factor varied with the stage of the annual cycle. The levels of NO3 −, K+, Mg2+, and Ca2+ followed the same pattern during the growing cycle, with both methods. Regression analysis resulted in high determination coefficients for NO3 − (R2 = 0.85), K+ (R2 = 0.86), Mg2+ (R2 = 0.81) and Ca2+ (R2 = 0.95), between RQflex® and laboratory methods. No equivalent relation was found for ammonium and phosphate determination. These tests can be useful tools for rational fertilization management, mainly in high-density apple orchards. The calcium content in 45 DAFB leaves correlated well with the calcium content in sap at the same timing
- Rapid Sap Nutrient Analysis Methods in Malus Domestica Borkh Cv. ‘Gala’Publication . Almeida, Patrícia da Costa; Oliveira, Cristina M.; Mota, Mariana; Ribeiro, HenriqueSap quick tests are well established in vegetable crops. However, there is less equivalent investigation for perennial crops, such as apple trees. Accessing the nutrient content, as opposed to the foliar analysis, would increase the opportunity of adjusting the fertilization, along the growing cycle. This work evaluates the relation between the NO3 −, K+, Ca2+, Mg2+, PO4 3- and NH4 + contents in apple petiole sap, measured with the RQflex® test strips and laboratory methods to assess the possibility of using this tool with accuracy in in-situ sap analysis. Petiole samples were collected from six apple tree orchards, frozen and pressed. Dilution was mandatory for all nutrient determination, except NO3 −. The dilution factor varied with the stage of the annual cycle. The levels of NO3 −, K+, Mg2+, and Ca2+ followed the same pattern during the growing cycle, with both methods. Regression analysis resulted in high determination coefficients for NO3 − (R2 = 0.85), K+ (R2 = 0.86), Mg2+ (R2 = 0.81) and Ca2+ (R2 = 0.95), between RQflex® and laboratory methods. No equivalent relation was found for ammonium and phosphate determination. These tests can be useful tools for rational fertilization management, mainly in high-density apple orchards. The calcium content in 45 DAFB leaves correlated well with the calcium content in sap at the same timing
- A step towards the production of manure-based fertilizers: Disclosing the effects of animal species and slurry treatment on their nutrients content and availabilityPublication . Prado, Joana; Ribeiro, Henrique; Alvarenga, Paula; Fangueiro, DavidIn a context of climate change and circular economy, it is fundamental to produce animal manure-based fertilizers attractive to farmers who are reluctant to use heterogeneous materials, poorly characterized and with unbalanced nutrient contents, namely N:P ratios different from plant requirements. Hence, the aim of the present study was to assess the potential of different treated and non-treated animal manures to produce tailor-made manure-based fertilizers with known N:P ratios. Seven manures were considered: cattle, caprine and poultry solid manures, two contrasting cattle slurries and two contrasting swine slurries. Slurries were further treated by solid-liquid separation, acidification, and acidification followed by solid-liquid separation. Raw and treated manures were fully characterized to assess the effect of manure type and treatment on their nutrient contents and N:P ratios, to incorporate them in manure-based fertilizers with selected N:P ratio. The present results were essential to support the manure-based fertilizer production and identify which materials could be considered: it appears clearly that the pig slurry with all stages of production and the correspondent liquid fraction cannot be used due to their low nutrients content. It was shown that the solid fractions rich in P and with a NT:N–NH4+ ratio close to 1 are an excellent material to prepare manure-based fertilizers. Acidification, combined with solidliquid separation, produced a liquid fraction rich in both N and P and with a NT:PT ratio close to 1, with the additional benefit of a slow N-release material. Some of the manures or sub-products obtained after treatment provided the usual N:P ratio required by farmers and could be used directly as substitutes of mineral fertilizers. Nevertheless, the blending of some of these materials has a stronger potential to the production of specific manure-based fertilizer
- Assessment of the agronomic value of manure-based fertilizersPublication . Prado, Joana; Fangueiro, David; Alvarenga, Paula; Ribeiro, HenriqueProducing manure-based fertilizers (MBFs) with specific nutrient ratios is a solution to overpassing the imbalance of nitrogen and phosphorus in manures, and a way to recycle manure’s nutrients, promoting sustainable agricultural practices. Several MBFs with different tailored N:P ratios (0.5:1, 1:1 and 2:1) were produced to determine their agronomic value in a pot experiment with oat (0.5:1 ratio: cattle manure with pig slurry (CaM+PiS), cattle manure with poultry manure (CaM+PoM) and poultry manure with superphosphate (PoM+SP); 1:1 ratio: poultry manure with cattle slurry (PoM+CaS) and poultry manure with pig slurry (PoM+PiS); 2:1 ratio: cattle slurry with the liquid fraction of cattle slurry (CaS+CaS-LIQ), pig slurry with the liquid fraction of pig slurry (PiS+PiS-LIQ) and poultry manure with urea (PoM+U)). The performance of these MBFs was compared with conventional mineral fertilizers (MFs) in sandy soils (Haplic Arenosols) with different nutrient requirements. Oat fertilized with PoM+SP (0.5:1) and PoM+PiS (1:1) led to yields similar to those obtained with the use of MFs (6.3 and 7.2 mg DM, respectively). The MBFs PoM+SP and PoM+PiS, as well as PiS+PiS-LIQ (2:1), were agronomically equivalent to the MFs. N uptake with those MBFs was equivalent to that obtained with the MFs. Replacing MFs with MBFs in the basal fertilization of oat was demonstrated to be a solution to turn agriculture more sustainable by recycling nutrients efficiently
- Development of new preventive strategies for pine pitch canker caused by Fusarium circinatum in irrigation water and evaluation in a real nursery contextPublication . Fernandes, Luís; Paiva, Diana; Roxo, Ivo; Fernandes, Ana Rita; Ribeiro, Dina; Ribeiro, Henrique; Portugal, AntónioFusarium circinatum is one of the many threats to forests and Pinus nurseries all over the world, being classified as a quarantine organism by several organizations and governing bodies, such as the European and Mediterranean Plant Protection Organization (EPPO) and the European Union (EU), with associated phytosanitary measures in place to prevent its spread through the various means of dispersal. One such means of dispersal is the water used for irrigation in nurseries, which can contain fungal propagules. Three different treatments, namely, Desogerme, Intra Hydrocare and sodium hypochlorite (NaClO), were tested for their efficacy in eliminating F. circinatum spores in water at several concentrations. Those that showed 100% disinfection rates were selected for further assays regarding seed germination and water quality impact. From these studies, Desogerme 1% and Intra Hydrocare 4% were then selected for large-scale seed germination and plant certification assays in nurseries, where they showed promising results in regard to the prevention of infections in nurseries, and in this way, contribute to the efforts of mitigating this disease.
- Nutrient potential leachability in a sandy soil amended with manure-based fertilisersPublication . Prado, Joana; Alvarenga, Paula; Ribeiro, Henrique; Fangueiro, DavidThe application of manure-based fertilisers (MBFs) is considered an important practice for achieving agricultural sustainability. However, the potential losses of nutrients to the environment need to be thoroughly evaluated. This study aimed to assess nutrients’ potential leachability from a sandy soil, fertilised with MBFs produced by mixing manure from one single animal species with N- or P-mineral fertilisers, to achieve target N:P ratios (1:1, 2:1 and 0.5:1). MBFs were prepared by combining pig slurry, cattle slurry or poultry manure with N- and P-mineral fertilisers, or slurryderived materials, obtained by solid–liquid separation. A leaching experiment was set-up in soil columns treated with MBFs, for 59 days, with seven leaching events. Poultry manure application to soil led to higher potential N leaching, while pig slurry induced higher P leaching. All 2:1 MBFs decreased P leaching, relative to the original manure, with the higher reduction (52%) being observed for pig slurry with urea. The addition of urea to poultry manure also diminished its potential for N leaching. The behaviour of P-enriched materials, pig slurry solid fraction and both 0.5:1 MBFs obtained with phosphoric acid addition showed a higher risk of P leaching, while the use of superphosphate as a P-mineral source decreased the risk of P leaching. Concluding, it is possible to use specific MBFs, enriched with N and P from mineral sources, and have lower N and P leaching potential, reducing the risks associated with manure soil application, while increasing their interest as alternative fertilisers.
- Evaluation of the fertilizer potential of Chlorella vulgaris and Scenedesmus obliquus grown in agricultural drainage water from maize fieldsPublication . Alvarenga, Paula; Martins, M.; Ribeiro, Henrique; Mota, M.; Guerra, I.; Cardoso, H.; Silva, J. L.Producing microalgae with agricultural drainage water (ADW) allows recycling water and nutrients, with the produc- tion of a biofertilizer, avoiding receiving waters' contamination. Chlorella vulgaris and Scenedesmus obliquus were culti- vated using ADW and standard media supplementation and presented higher productivities, relatively to the control industrial growth medium (using freshwater). Selected strains were grown outdoors in pilot flat panel photobioreactors, reaching 2.20 g L−1 for S. obliquus and 1.15 g L−1 for C. vulgaris, and degrading herbicides in the ADW to non-quantifiable concentrations. The potential of the C. vulgaris and S. obliquus suspensions to replace 50% of nitrogen (N) mineral fertilization of lettuce (0.5 g pot−1) was evaluated through a pot trial, also using a 2-times (1.0 g pot−1) and 5-times (2.5 g pot−1) higher dose, applied 31 days before lettuce transplanting. Even the lower dose of N, applied via C. vulgaris or S. obliquus suspensions, was able to provide significantly higher lettuce fresh matter yield, relatively to the mineral fertilized control. Soil enzymatic activities were improved, with significantly higher de- hydrogenase, β-glucosidase, and acid phosphatase activities for the 2.5 g pot−1 dose, more marked for S. obliquus, which was also able to increase soil organic matter content. Both the non-fertilized control and microalgae fertilized pots led to similar soil electrical conductivities, 3-fold lower than in the N-mineral fertilized pots, evidencing the capac- ity of microalgae fertilizers to avoid soil secondary salinization. Results suggest benefits from using ADW from maize cultivation to produce C. vulgaris or S. obliquus suspensions, that can be further used as liquid organic slow-release fertilizer.
- Evaluation of the fertilizer potential of Chlorella vulgaris and Scenedesmus obliquus grown in agricultural drainage water from maize fieldsPublication . Alvarenga, Paula; Martins, Marta; Ribeiro, Henrique; Mota, Mariana; Guerra, Inês; Cardoso, Helena; Silva, Joana LaranjeiraProducing microalgae with agricultural drainage water (ADW) allows recycling water and nutrients, with the production of a biofertilizer, avoiding receiving waters' contamination. Chlorella vulgaris and Scenedesmus obliquus were cultivated using ADW and standard media supplementation and presented higher productivities, relatively to the control industrial growth medium (using freshwater). Selected strains were grown outdoors in pilot flat panel photobioreactors, reaching 2.20 g L−1 for S. obliquus and 1.15 g L−1 for C. vulgaris, and degrading herbicides in the ADW to non-quantifiable concentrations. The potential of the C. vulgaris and S. obliquus suspensions to replace 50% of nitrogen (N) mineral fertilization of lettuce (0.5 g pot−1) was evaluated through a pot trial, also using a 2-times (1.0 g pot−1) and 5-times (2.5 g pot−1) higher dose, applied 31 days before lettuce transplanting. Even the lower dose of N, applied via C. vulgaris or S. obliquus suspensions, was able to provide significantly higher lettuce fresh matter yield, relatively to themineral fertilized control. Soil enzymatic activitieswere improved, with significantly higher dehydrogenase, β-glucosidase, and acid phosphatase activities for the 2.5 g pot−1 dose, more marked for S. obliquus, which was also able to increase soil organic matter content. Both the non-fertilized control and microalgae fertilized pots led to similar soil electrical conductivities, 3-fold lower than in theN-mineral fertilized pots, evidencing the capacity of microalgae fertilizers to avoid soil secondary salinization. Results suggest benefits from using ADW from maize cultivation to produce C. vulgaris or S. obliquus suspensions, that can be further used as liquid organic slow-release fertilizer.
- Análise de nutrientes em "seiva" de macieira (Malus domestica Borkh cv. Gala)Publication . Almeida, Patrícia da Costa; Mota, Mariana; Ribeiro, Henrique; Oliveira, Cristina M.