Browsing by Author "Mendes, Ana"
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- Antiretroviral Drug Resistance Surveillance among Treatment-Naive Human Immunodeficiency Virus Type 1-Infected Individuals in AngolaPublication . Bartolo, Ines; Rocha, Cheila; Bartolomeu, Jose; Gama, Antonio; Fonseca, Marlene; Mendes, Ana; Cristina, Filipa; Thamm, Sven; Epalanga, Marta; Silva, Patricia Cavaco; Taveira, NunoThe prevalence of transmitted human immunodeficiency virus type 1 drug resistance in Angola in 2001 in 196 untreated patients was investigated. All subtypes were detected, along with unclassifiable and complex recombinant strains. Numerous new polymorphis. - Fundacao GlaxoSmithKline das Ciencias da Saude and Fundacao para a Ciencia e Tecnologia, Portugal [PTDC/SAU-FCF/67673/2006]; Fundacao para a Ciencia e Tecnologia, Portugal. - This work was supported by grants from Fundacao GlaxoSmithKline das Ciencias da Saude and Fundacao para a Ciencia e Tecnologia, Portugal (PTDC/SAU-FCF/67673/2006). Ines Bartolo and Cheila Rocha are supported by Ph. D. grants from Fundacao para a Ciencia e
- Antiretroviral drug resistance surveillance among treatment-naive human immunodeficiency virus type 1-infected individuals in Angola: Evidence for low level of transmitted drug resistancePublication . Bártolo, Inês; Rocha, Cheila; Bartolomeu, José; Gama, António; Fonseca, Marlene; Mendes, Ana; Cristina, Filipa; Thamm, Sven; Epalanga, Marta; Silva, Patrícia Cavaco; Taveira, NunoThe prevalence of transmitted human immunodeficiency virus type 1 drug resistance in Angola in 2001 in 196 untreated patients was investigated. All subtypes were detected, along with unclassifiable and complex recombinant strains. Numerous new polymorphisms were identified in the reverse transcriptase and protease. Two (1.6%) unrelated patients harbored nucleoside reverse transcriptase inhibitor- and nonnucleoside reverse transcriptase inhibitor-resistant viruses (mutations: M41L, D67N, M184V, L210W, T215Y or T215F, and K103N). Continued surveillance of drug resistance is required for maximization of ART efficacy in Angola.
- High genetic diversity of human immunodeficiency virus type 1 in AngolaPublication . Bártolo, Inês; Epalanga, Marta; Bartolomeu, José; Fonseca, Marlene; Mendes, Ana; Gama, António; Taveira, NunoTo investigate which HIV-1 genetic forms are circulating in Angola, we have determined the gag and/or env genotypes of 48 isolates from patients living in Cabinda and Luanda provinces. The following subtypes were identified: A1 (18 samples, 38%), C (7, 15%), H (5, 10%), J (3, 6%), G (2, 4%), A2 (2, 4%), F1 (1, 2%), and D (1, 2%). The env gene fragment was untypable in one sample. Discordant subtype classifications in the gag and env genes were found in eight (17%) samples. There were six different recombination patterns (gag/env): A1/H (3, 6%), A1/G (1, 2%), C/A2 (1, 2%), F1/B (1, 2%), G/B (1, 2%), and G/H (1, 2%). The A1/H recombinant may represent a new circulating recombinant form. The marked genetic heterogeneity of HIV-1 in Angola has important implications for vaccine development.
- Highly divergent subtypes and new recombinant forms prevail in the HIV/AIDS epidemic in AngolaPublication . Bartolo, Ines; Rocha, Cheila; Bartolomeu, Jose; Gama, Antonio; Marcelino, Rute; Fonseca, Marlene; Mendes, Ana; Epalanga, Marta; Silva, Patricia Cavaco; Taveira, NunoAngola, located in South-Western Africa, has a remarkably low HIV/AIDS prevalence in the adult population (3.7%). It is bordered in the North by the Democratic Republic of Congo (DRC) and Republic of Congo that are at the origin of human HIV-1 infections.
- Highly divergent subtypes and new recombinant forms prevail in the HIV/AIDS epidemic in Angola: New insights into the origins of the AIDS pandemicPublication . Bártolo, Inês; Rocha, Cheila; Bartolomeu, José; Gama, António; Marcelino, Rute; Fonseca, Marlene; Mendes, Ana; Epalanga, Marta; Silva, Patrícia Cavaco; Taveira, NunoAngola, located in South-Western Africa, has a remarkably low HIV/AIDS prevalence in the adult population (3.7%). It is bordered in the North by the Democratic Republic of Congo (DRC) and Republic of Congo that are at the origin of human HIV-1 infections. It is, therefore, likely that HIV-1 strains circulating in Angola are genetically diverse and representative of the origin of the HIV/AIDS epidemic. The aim of this work was to investigate in detail the genetic diversity and molecular epidemiology of HIV-1 in Angola. Almost 400 sequences were obtained from the gag (p17), pol (PR and RT) and/or env (C2C3) genes of 159 HIV-1 infected patients living in eight provinces of Angola (Benguela, Cabinda, Cuanza Norte, Luanda, Lunda Norte, Malange, Uíge, and Zaire) and their genotype was determined by phylogenetic analyses. Gene regions representing all HIV-1 group M clades were found as well as unclassifiable sequences. In env and pol (RT), two groups of sequences forming distinct sub-clusters within the subtype A radiation were found and may define new A5 and A6 sub-subtypes. Recombinant forms were found in almost half (47.1%) of the patients of which 36.0% were second-generation recombinants. Fifty-eight different patterns of recombination were found. The A subtype, including CRF02_AG, was represented in most recombinant viruses. Epidemiological data suggests that the AIDS epidemic in Angola has probably started as early as 1961, the major cause being the independence war, and spread to Portugal soon thereafter. The extraordinary degree of HIV-1 group M genetic diversity and evolution in Angola may pose unprecedented challenges to diagnostic, treatment and prevention of HIV-1 infection.
- Método das preferências visuaisPublication . Castel-Branco, Cristina; Soares, Ana Luísa; Arsénio, Pedro; Mesquita, Sandra; Mendes, Ana; Doria, Carolina; Silva, Joana Santos; Santiago, Raquel
- A spatial stream-network approach assists in managing the remnant genetic diversity of riparian forestsPublication . Rodríguez-González, Patrícia Maria; Garcia, Cristina; Albuquerque, António; Monteiro-Henriques, Tiago; Faria, Carla; Guimarães, Joana B.; Mendonça, Diogo; Simões, Fernanda; Ferreira, Teresa; Mendes, Ana; Matos, José; Almeida, Maria HelenaQuantifying the genetic diversity of riparian trees is essential to understand their chances to survive hydroclimatic alterations and to maintain their role as foundation species modulating fluvial ecosystem processes. However, the application of suitable models that account for the specific dendritic structure of hydrographic networks is still incipient in the literature. We investigate the roles of ecological and spatial factors in driving the genetic diversity of Salix salviifolia, an Iberian endemic riparian tree, across the species latitudinal range. We applied spatial stream-network models that aptly integrate dendritic features (topology, directionality) to quantify the impacts of multiple scale factors in determining genetic diversity. Based on the drift hypothesis, we expect that genetic diversity accumulates downstream in riparian ecosystems, but life history traits (e.g. dispersal patterns) and abiotic or anthropogenic factors (e.g. drought events or hydrological alteration) might alter expected patterns. Hydrological factors explained the downstream accumulation of genetic diversity at the intermediate scale that was likely mediated by hydrochory. The models also suggested upstream gene flow within basins that likely occurred through anemophilous and entomophilous pollen and seed dispersal. Higher thermicity and summer drought were related to higher population inbreeding and individual homozygosity, respectively, suggesting that increased aridity might disrupt the connectivity and mating patterns among and within riparian populations
- A spatial stream-network approach assists in managing the remnant genetic diversity of riparian forestsPublication . Rodríguez-González, Patricia María; García, Cristina; Albuquerque, António; Monteiro-Henriques, Tiago; Faria, Carla; Guimarães, Joana B.; Mendonça, Diogo; Simões, Fernanda; Ferreira, Maria Teresa; Mendes, Ana; Matos, José; Almeida, Maria HelenaQuantifying the genetic diversity of riparian trees is essential to understand their chances to survive hydroclimatic alterations and to maintain their role as foundation species modulating fluvial ecosystem processes. However, the application of suitable models that account for the specific dendritic structure of hydrographic networks is still incipient in the literature. We investigate the roles of ecological and spatial factors in driving the genetic diversity of Salix salviifolia, an Iberian endemic riparian tree, across the species latitudinal range. We applied spatial stream-network models that aptly integrate dendritic features (topology, directionality) to quantify the impacts of multiple scale factors in determining genetic diversity. Based on the drift hypothesis, we expect that genetic diversity accumulates downstream in riparian ecosystems, but life history traits (e.g. dispersal patterns) and abiotic or anthropogenic factors (e.g. drought events or hydrological alteration) might alter expected patterns. Hydrological factors explained the downstream accumulation of genetic diversity at the intermediate scale that was likely mediated by hydrochory. The models also suggested upstream gene flow within basins that likely occurred through anemophilous and entomophilous pollen and seed dispersal. Higher thermicity and summer drought were related to higher population inbreeding and individual homozygosity, respectively, suggesting that increased aridity might disrupt the connectivity and mating patterns among and within riparian populations.
- The portuguese severe asthma registry : development, features, and data sharing policiesPublication . Sousa, Ana Sá; Fonseca, João Almeida; Pereira, Ana Margarida; Ferreira, Ana; Arrobas, Ana; Mendes, Ana; Drummond, Marta; Videira, Wanda; Costa, Tiago; Farinha, Pedro; Soares, José; Rocha, Pedro; Todo-Bom, Ana; Sokolova, Anna; Costa, António; Fernandes, Beatriz; Loureiro, Carla Chaves; Longo, Cecília; Pardal, Cecília; Costa, Célia; Cruz, Cíntia; Loureiro, Cláudia Chaves; Lopes, Cristina; Mesquita, Duarte; Faria, Emília; Magalhães, Eunice; Menezes, Fernando; Todo-Bom, Filipa; Carvalho, Francisca; Regateiro, Frederico S.; Falcão, Helena; Fernandes, Ivone; Marques, João Gaspar; Viana, Jorge; Ferreira, José; Silva, José Manuel; Simão, Laura; Almeida, Leonor; Fernandes, Lígia; Ferreira, Lurdes; van Zeller, Mafalda; Quaresma, Márcia; Castanho, Margarida; André, Natália; Cortesão, Nuno; Pinto, Paula Leiria; Pinto, Paula; Rosa, Paula; Martins, Pedro Carreiro; Gerardo, Rita; Silva, Rui; Lucas, Susana; Almeida, Teresa; Calvo, TeresaThe Portuguese Severe Asthma Registry (Registo de Asma Grave Portugal, RAG) was developed by an open collaborative network of asthma specialists. RAG collects data from adults and pediatric severe asthma patients that despite treatment optimization and adequate management of comorbidities require step 4/5 treatment according to GINA recommendations. In this paper, we describe the development and implementation of RAG, its features, and data sharing policies. The contents and structure of RAG were defined in a multistep consensus process. A pilot version was pretested and iteratively improved. The selection of data elements for RAG considered other severe asthma registries, aiming at characterizing the patient's clinical status whilst avoiding overloading the standard workflow of the clinical appointment. Features of RAG include automatic assessment of eligibility, easy data input, and exportable data in natural language that can be pasted directly in patients' electronic health record and security features to enable data sharing (among researchers and with other international databases) without compromising patients' confidentiality. RAG is a national web-based disease registry of severe asthma patients, available at asmagrave.pt. It allows prospective clinical data collection, promotes standardized care and collaborative clinical research, and may contribute to inform evidence-based healthcare policies for severe asthma.