The challenge of peptide proteolytic stability studies: scarce data, difficult readability, and the need for harmonization

Cavaco, Marco; Andreu, David; Castanho, Miguel A. R. B.

http://hdl.handle.net/10451/57398

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Authors

Cavaco, Marco

Andreu, David

Castanho, Miguel A. R. B.

Abstract(s)

Proteolytic stability assessment is increasingly viewed as a fundamental component of peptide characterization, arguably of comparable importance as efficacy and toxicity data. A literature survey over the last decade reveals steady growth in the stability information available. However, it also uncovers two significant problems that hinder proper data comparison: 1) the use of different stability assays, and 2) the differences in how stability information is reported. In this Viewpoint, we present results from a database meta-analysis as well as concerns about the stability assessments published so far. We also suggest guidelines for a proper discussion between experts in the field on how to improve data readability so that peptide stability, an often-missing parameter in older literature, is adequately reported to take maximum advantage of it.