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Advisor(s)
Abstract(s)
Background: The endometrium is a heterogeneous tissue composed of luminal epithelial (LE), glandular epithelial (GE), and stromal cells (ST), experiencing progesterone regulated dynamic changes during the estrous cycle. In the cow, this regulation at the transcriptomic level was only evaluated in the whole tissue. This study describes specifc gene expression in the three types of cells isolated from endometrial biopsies following laser capture microdissection and the transcriptome changes induced by progesterone in GE and ST cells. Results: Endometrial LE, GE, and ST cells show specifc transcriptomic profles. Most of the diferentially expressed genes (DEGs) in response to progesterone are cell type-specifc (96%). Genes involved in cell cycle and nuclear divi sion are under-expressed in the presence of progesterone in GE, highlighting the anti-proliferative action of pro gesterone in epithelial cells. Elevated progesterone concentrations are also associated with the under-expression of estrogen receptor 1 (ESR1) in GE and oxytocin receptor (OXTR) in GE and ST cells. In ST cells, transcription factors such as SOX17 and FOXA2, known to regulate uterine epithelial-stromal cross-talk conveying to endometrial receptivity, are over-expressed under progesterone infuence. Conclusions: The results from this study show that progesterone regulates endometrial function in a cell type-spe cifc way, which is independent of the expression of its main receptor PGR. These novel insights into uterine physiol ogy present the cell compartment as the physiological unit rather than the whole tissue.
Description
Research Areas: Biotechnology & Applied Microbiology ; Genetics & Heredity
Keywords
Transcriptome Progesterone Endometrium LCM RNA-seq
Pedagogical Context
Citation
Pereira G, Guo YZ, Silva E, Bevilacqua C, Charpigny G, Lopes-da-Costa L, Humblot P. 2022. Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types. BMC Genomics, 23(1):82. DOI 10.1186/s12864-022-08323-z
Publisher
BMC