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Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types

2022-01-27Journal article Open access
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dc.contributor.authorPereira, Gonçalo
dc.contributor.authorGuo, YZ
dc.contributor.authorSilva, E
dc.contributor.authorBevilacqua, C
dc.contributor.authorCharpigny, G
dc.contributor.authorLopes-da-Costa, Luís
dc.contributor.authorHumblot, P
dc.date.accessioned2022-04-27T15:15:25Z
dc.date.available2022-04-27T15:15:25Z
dc.date.issued2022-01-27
dc.descriptionResearch Areas: Biotechnology & Applied Microbiology ; Genetics & Hereditypt_PT
dc.description.abstractBackground: The endometrium is a heterogeneous tissue composed of luminal epithelial (LE), glandular epithelial (GE), and stromal cells (ST), experiencing progesterone regulated dynamic changes during the estrous cycle. In the cow, this regulation at the transcriptomic level was only evaluated in the whole tissue. This study describes specifc gene expression in the three types of cells isolated from endometrial biopsies following laser capture microdissection and the transcriptome changes induced by progesterone in GE and ST cells. Results: Endometrial LE, GE, and ST cells show specifc transcriptomic profles. Most of the diferentially expressed genes (DEGs) in response to progesterone are cell type-specifc (96%). Genes involved in cell cycle and nuclear divi sion are under-expressed in the presence of progesterone in GE, highlighting the anti-proliferative action of pro gesterone in epithelial cells. Elevated progesterone concentrations are also associated with the under-expression of estrogen receptor 1 (ESR1) in GE and oxytocin receptor (OXTR) in GE and ST cells. In ST cells, transcription factors such as SOX17 and FOXA2, known to regulate uterine epithelial-stromal cross-talk conveying to endometrial receptivity, are over-expressed under progesterone infuence. Conclusions: The results from this study show that progesterone regulates endometrial function in a cell type-spe cifc way, which is independent of the expression of its main receptor PGR. These novel insights into uterine physiol ogy present the cell compartment as the physiological unit rather than the whole tissue.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPereira G, Guo YZ, Silva E, Bevilacqua C, Charpigny G, Lopes-da-Costa L, Humblot P. 2022. Progesterone differentially affects the transcriptomic profiles of cow endometrial cell types. BMC Genomics, 23(1):82. DOI 10.1186/s12864-022-08323-zpt_PT
dc.identifier.doi10.1186/s12864-022-08323-zpt_PT
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/10400.5/24177
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBMCpt_PT
dc.relationalterado para: “Cytological endometritis in dairy cattle: news insights into pathogenesis, diagnosis and therapy”. Effects of Bovine Venereal Campylobacteriosis and Bovine Viral Diarrohea interaction on beef herd reproductive efficiency
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationExploring resolving mechanisms in cow persistent uterine and mammary inflammation
dc.relation.publisherversionhttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-022-08323-zpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectTranscriptomept_PT
dc.subjectProgesteronept_PT
dc.subjectEndometriumpt_PT
dc.subjectLCMpt_PT
dc.subjectRNA-seqpt_PT
dc.titleProgesterone differentially affects the transcriptomic profiles of cow endometrial cell typespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlealterado para: “Cytological endometritis in dairy cattle: news insights into pathogenesis, diagnosis and therapy”. Effects of Bovine Venereal Campylobacteriosis and Bovine Viral Diarrohea interaction on beef herd reproductive efficiency
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleExploring resolving mechanisms in cow persistent uterine and mammary inflammation
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F130923%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-CVT%2F6932%2F2020/PT
oaire.citation.conferencePlaceLondon, ENGLANDpt_PT
oaire.citation.titleBMC Genomicspt_PT
oaire.citation.volume23(1):82pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
person.familyNameTomé Sousa Silva
person.familyNameLopes-da-Costa
person.givenNameMaria Elisabete
person.givenNameLuís
person.identifier.ciencia-id651A-9A4A-1CE0
person.identifier.ciencia-id0A1F-AC68-0919
person.identifier.orcid0000-0002-2332-7642
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicatione2277e06-ec5b-4f68-8a39-4e4e27389e9c
relation.isAuthorOfPublication.latestForDiscoverycb5b1c30-fb12-4e71-a79d-3e7593037784
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