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Enhance 2-phenylethanol production by the bacterium Acinetobacter soli ANG344B

2025Dissertação de mestrado Acesso aberto
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dc.contributor.advisorTorres,Cristiana Andreia Vieira
dc.contributor.advisorLourenço,Artur Pedro Duarte Reis Bastos
dc.contributor.authorSilva,Rodrigo Manuel de Oliveira Quaresma da
dc.contributor.institutionFaculty of Sciences
dc.date.accessioned2026-02-12T14:45:03Z
dc.date.available2026-02-12T14:45:03Z
dc.date.issued2025
dc.descriptionTese de Mestrado, Microbiologia Aplicada, 2025, Universidade de Lisboa, Faculdade de Ciências
dc.description.abstract2-Phenylethanol (2-PE) is a high-value aromatic alcohol with applications in the food, cosmetic, and pharmaceutical industries due to its rose-like fragrance and antimicrobial properties. This study explored the biotechnological production of 2-PE using Acinetobacter soli ANG344B, a novel bacterial strain harboring a complete Ehrlich pathway. Cultivation was performed in both shake-flasks and bioreactors, with and without in situ product removal (ISPR) strategies, namely adsorption onto Amberlite XAD-4 resin and liquid–liquid extraction (LLE) with soybean oil. Shake-flask controls reached modest titers (1.2 g/L), while bioreactor operation slightly improved production (1.7 g/L). Resin-based ISPR drastically increased 2-PE titers to 28.45 g/L (48 h) and 74.49 g/L (72 h), obtained by desorption of 2-PE from the resin with ethanol and its evaporation afterwards, confirming the capacity of hydrophobic polymeric resins to alleviate product toxicity and extend metabolic activity. In contrast, LLE with soybean oil supported biomass accumulation but achieved only 2.4 g/L of 2-PE, reflecting poor partitioning efficiency. Aromatic profiling revealed that resin systems favored a broader metabolite spectrum, while resin-free assays were dominated by 2-PE, suggesting saturation effects at inhibitory concentrations. These results validate A. soli ANG344B as a promising bacterial chassis for natural 2-PE production and highlight adsorption resins as superior ISPR tools compared to vegetable oils. Future work should focus on fed-batch operation, resin regeneration cycles, and metabolic engineering of redox balance to further improve titers and yields. Integration with green solvents such as deep eutectic systems also represents a promising direction for sustainable aroma recovery.en
dc.formatapplication/pdf
dc.identifier.tid204173957
dc.identifier.urihttp://hdl.handle.net/10400.5/117039
dc.language.isoeng
dc.subject2-Phenylethanol
dc.subjectAcinetobacter soli ANG344B
dc.subjectIn situ product removal
dc.subjectAmberlite XAD-4
dc.subjectGreen solvents
dc.titleEnhance 2-phenylethanol production by the bacterium Acinetobacter soli ANG344Ben
dc.typemaster thesis
dspace.entity.typePublication
rcaap.rightsopenAccess

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