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Enhance 2-phenylethanol production by the bacterium Acinetobacter soli ANG344B
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Resumo(s)
2-Phenylethanol (2-PE) is a high-value aromatic alcohol with applications in the food, cosmetic, and pharmaceutical industries due to its rose-like fragrance and antimicrobial properties. This study explored the biotechnological production of 2-PE using Acinetobacter soli ANG344B, a novel bacterial strain harboring a complete Ehrlich pathway. Cultivation was performed in both shake-flasks and bioreactors, with and without in situ product removal (ISPR) strategies, namely adsorption onto Amberlite XAD-4 resin and liquid–liquid extraction (LLE) with soybean oil. Shake-flask controls reached modest titers (1.2 g/L), while bioreactor operation slightly improved production (1.7 g/L). Resin-based ISPR drastically increased 2-PE titers to 28.45 g/L (48 h) and 74.49 g/L (72 h), obtained by desorption of 2-PE from the resin with ethanol and its evaporation afterwards, confirming the capacity of hydrophobic polymeric resins to alleviate product toxicity and extend metabolic activity. In contrast, LLE with soybean oil supported biomass accumulation but achieved only 2.4 g/L of 2-PE, reflecting poor partitioning efficiency. Aromatic profiling revealed that resin systems favored a broader metabolite spectrum, while resin-free assays were dominated by 2-PE, suggesting saturation effects at inhibitory concentrations. These results validate A. soli ANG344B as a promising bacterial chassis for natural 2-PE production and highlight adsorption resins as superior ISPR tools compared to vegetable oils. Future work should focus on fed-batch operation, resin regeneration cycles, and metabolic engineering of redox balance to further improve titers and yields. Integration with green solvents such as deep eutectic systems also represents a promising direction for sustainable aroma recovery.
Descrição
Tese de Mestrado, Microbiologia Aplicada, 2025, Universidade de Lisboa, Faculdade de Ciências
Palavras-chave
2-Phenylethanol Acinetobacter soli ANG344B In situ product removal Amberlite XAD-4 Green solvents