Browsing by Author "Romano, A."
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- ARIA 2016: care pathways implementing emerging technologies for predictive medicine in rhinitis and asthma across the life cyclePublication . Bousquet, J.; Hellings, P. W.; Agache, I.; Bedbrook, A.; Bachert, C.; Bergmann, K. C.; Bewick, M.; Bindslev-Jensen, C.; Bosnic-Anticevitch, S.; Bucca, C.; Caimmi, D. P.; Camargos, P. A. M.; Canonica, G. W.; Casale, T.; Chavannes, N. H.; Cruz, A. A.; De Carlo, G.; Dahl, R.; Demoly, P.; Devillier, P.; Fonseca, J.; Fokkens, W. J.; Guldemond, N. A.; Haahtela, T.; Illario, M.; Just, J.; Keil, T.; Klimek, L.; Kuna, P.; Larenas-Linnemann, D.; Morais-Almeida, M.; Mullol, J.; Murray, R.; Naclerio, R.; O’Hehir, R. E.; Papadopoulos, N. G.; Pawankar, R.; Potter, P.; Ryan, D.; Samolinski, B.; Schunemann, H. J.; Sheikh, A.; Simons, F. E. R.; Stellato, C.; Todo-Bom, A.; Tomazic, P. V.; Valiulis, A.; Valovirta, E.; Ventura, M. T.; Wickman, M.; Young, I.; Yorgancioglu, A.; Zuberbier, T.; Aberer, W.; Akdis, C. A.; Akdis, M.; Annesi-Maesano, I.; Ankri, J.; Ansotegui, I. J.; Anto, J. M.; Arnavielhe, S.; Asarnoj, A.; Arshad, H.; Avolio, F.; Baiardini, I.; Bárbara, Cristina; Barbagallo, M.; Bateman, E. D.; Beghé, B.; Bel, E. H.; Bennoor, K. S.; Benson, M.; Białoszewski, A. Z.; Bieber, T.; Bjermer, L.; Blain, H.; Blasi, F.; Boner, A. L.; Bonini, M.; Bonini, S.; Bosse, I.; Bouchard, J.; Boulet, L. P.; Bourret, R.; Bousquet, P. J.; Braido, F.; Briggs, A. H.; Brightling, C. E.; Brozek, J.; Buhl, R.; Bunu, C.; Burte, E.; Bush, A.; Caballero-Fonseca, F.; Calderon, M. A.; Camuzat, T.; Cardona, V.; Carreiro-Martins, P.; Carriazo, A. M.; Carlsen, K. H.; Carr, W.; Cepeda Sarabia, A. M.; Cesari, M.; Chatzi, L.; Chiron, R.; Chivato, T.; Chkhartishvili, E.; Chuchalin, A. G.; Chung, K. F.; Ciprandi, G.; de Sousa, J. Correia; Cox, L.; Crooks, G.; Custovic, A.; Dahlen, S. E.; Darsow, U.; Dedeu, T.; Deleanu, D.; Denburg, J. A.; De Vries, G.; Didier, A.; Dinh-Xuan, A. T.; Dokic, D.; Douagui, H.; Dray, G.; Dubakiene, R.; Durham, S. R.; Du Toit, G.; Dykewicz, M. S.; Eklund, P.; El-Gamal, Y.; Ellers, E.; Emuzyte, R.; Farrell, J.; Fink Wagner, A.; Fiocchi, A.; Fletcher, M.; Forastiere, F.; Gaga, M.; Gamkrelidze, A.; Gemicioğlu, B.; Gereda, J. E.; van Wick, R. Gerth; González Diaz, S.; Grisle, I.; Grouse, L.; Gutter, Z.; Guzmán, M. A.; Hellquist-Dahl, B.; Heinrich, J.; Horak, F.; Hourihane, J. O’. B.; Humbert, M.; Hyland, M.; Iaccarino, G.; Jares, E. J.; Jeandel, C.; Johnston, S. L.; Joos, G.; Jonquet, O.; Jung, K. S.; Jutel, M.; Kaidashev, I.; Khaitov, M.; Kalayci, O.; Kalyoncu, A. F.; Kardas, P.; Keith, P. K.; Kerkhof, M.; Kerstjens, H. A. M.; Khaltaev, N.; Kogevinas, M.; Kolek, V.; Koppelman, G. H.; Kowalski, M. L.; Kuitunen, M.; Kull, I.; Kvedariene, V.; Lambrecht, B.; Lau, S.; Laune, D.; Le, L. T. T.; Lieberman, P.; Lipworth, B.; Li, J.; Lodrup Carlsen, K. C.; Louis, R.; Lupinek, C.; MacNee, W.; Magar, Y.; Magnan, A.; Mahboub, B.; Maier, D.; Majer, I.; Malva, J.; Manning, P.; De Manuel Keenoy, E.; Marshall, G. D.; Masjedi, M. R.; Mathieu-Dupas, E.; Maurer, M.; Mavale-Manuel, S.; Melén, E.; Melo-Gomes, E.; Meltzer, E. O.; Mercier, J.; Merk, H.; Miculinic, N.; Mihaltan, F.; Milenkovic, B.; Millot-Keurinck, J.; Mohammad, Y.; Momas, I.; Mösges, R.; Muraro, A.; Namazova-Baranova, L.; Nadif, R.; Neffen, H.; Nekam, K.; Nieto, A.; Niggemann, B.; Nogueira-Silva, L.; Nogues, M.; Nyembue, T. D.; Ohta, K.; Okamoto, Y.; Okubo, K.; Olive-Elias, M.; Ouedraogo, S.; Paggiaro, P.; Pali-Schöll, I.; Palkonen, S.; Panzner, P.; Papi, A.; Park, H. S.; Passalacqua, G.; Pedersen, S.; Pereira, A. M.; Pfaar, O.; Picard, R.; Pigearias, B.; Pin, I.; Plavec, D.; Pohl, W.; Popov, T. A.; Portejoie, F.; Postma, D.; Poulsen, L. K.; Price, D.; Rabe, K. F.; Raciborski, F.; Roberts, G.; Robalo-Cordeiro, C.; Rodenas, F.; Rodriguez-Mañas, L.; Rolland, C.; Roman Rodriguez, M.; Romano, A.; Rosado-Pinto, J.; Rosario, N.; Rottem, M.; Sanchez-Borges, M.; Sastre-Dominguez, J.; Scadding, G. K.; Scichilone, N.; Schmid-Grendelmeier, P.; Serrano, E.; Shields, M.; Siroux, V.; Sisul, J. C.; Skrindo, I.; Smit, H. A.; Solé, D.; Sooronbaev, T.; Spranger, O.; Stelmach, R.; Sterk, P. J.; Strandberg, T.; Sunyer, J.; Thijs, C.; Triggiani, M.; Valenta, R.; Valero, A.; van Eerd, M.; van Ganse, E.; van Hague, M.; Vandenplas, O.; Varona, L. L.; Vellas, B.; Vezzani, G.; Vazankari, T.; Viegi, G.; Vontetsianos, T.; Wagenmann, M.; Walker, S.; Wang, D. Y.; Wahn, U.; Werfel, T.; Whalley, B.; Williams, D. M.; Williams, S.; Wilson, N.; Wright, J.; Yawn, B. P.; Yiallouros, P. K.; Yusuf, O. M.; Zaidi, A.; Zar, H. J.; Zernotti, M. E.; Zhang, L.; Zhong, N.; Zidarn, M.The Allergic Rhinitis and its Impact on Asthma (ARIA) initiative commenced during a World Health Organization workshop in 1999. The initial goals were (1) to propose a new allergic rhinitis classification, (2) to promote the concept of multi-morbidity in asthma and rhinitis and (3) to develop guidelines with all stakeholders that could be used globally for all countries and populations. ARIA-disseminated and implemented in over 70 countries globally-is now focusing on the implementation of emerging technologies for individualized and predictive medicine. MASK [MACVIA (Contre les Maladies Chroniques pour un Vieillissement Actif)-ARIA Sentinel NetworK] uses mobile technology to develop care pathways for the management of rhinitis and asthma by a multi-disciplinary group and by patients themselves. An app (Android and iOS) is available in 20 countries and 15 languages. It uses a visual analogue scale to assess symptom control and work productivity as well as a clinical decision support system. It is associated with an inter-operable tablet for physicians and other health care professionals. The scaling up strategy uses the recommendations of the European Innovation Partnership on Active and Healthy Ageing. The aim of the novel ARIA approach is to provide an active and healthy life to rhinitis sufferers, whatever their age, sex or socio-economic status, in order to reduce health and social inequalities incurred by the disease.
- Effect of in vitro cold storage on surviving and proliferation of cork-oak (Quercus suber L.) culturesPublication . Romano, A.; Noronha, C.; Martins-Loução, M. A.Cork-oak cultures were stored in vitro without na intervening subculture, at 5º C in the dark for 5 months without loosing their capacity to regenerate when subcultured under standard conditions. The viability, proliferation rate and elongation of the explants were evaluated during 5 months of storage. Proliferation rate of stored cultures was observed during 5 months of storage. Proliferation rate of stored cultures was observed during the following 3 profileration cycles and compared to unstored controls of the same age. Shoot cultures survived with 100% viability and the proliferation rate was not statistically different from the controls. The results here described indicate the feasibility of establishing na in vitro active gene bank for Quercus suber. Cultivos de alcornoque han sido armacenados a 5º en oscuridad durante 5 meses sin ninguna intervención de subcultivo. Los cultivos no han perdido la capacidad de regeneración cuando cultivados en las condiciones estandardizadas. La viabilidad, la tasa de multiplicación y la alongación de los explantos han sido analizados durante 5 meses. La multiplicación de los cultivos almacenados se estudió durante los seguintes 3 ciclos de multiplicación y se comparo con los controlos sin almacenaje com la misma edad. Los cultivos han presentado 100% de viabilidad y la tasa de multiplicación no há sido estadisticamente diferente del control. Los resultados presentados demuestran la capacidad de establecer in vitro un banco de genes activo para el alcornoque (Quercus suber L.).
- Floral Analysis and Seasonal Dynamics of Mineral Levels in Carob Tree LeavesPublication . Custódio, L.; Correia, P.J.; Martins-Loução, M. A.; Romano, A.The mineral concentration of flowers and the seasonal fluctuation of macro- [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg)] and micronutrients [iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu)] in leaves of male, female, and hermaphroditic carob trees (Ceratonia siliqua L.) were studied. The nutrient dynamics were linked not only to phenological events, but also to the gender of the trees. The females were able to allocate more nutrients to leaves than male trees, even though male flowers were richer in particular elements such as N and Zn. The hermaphrodites supported the development of both inflorescences and fruits with a lower seasonal variation and a lower leaf nutrient concentration, as compared to the other genders, which may indicate a more efficient use of resources. Flowers had, in general, a higher concentration of N, P, and K, and a lower Mn concentration than in leaves. Flowers of the females had a lower nutrient concentration compared to males or hermaphrodites.
- Influence of growth regulators on shoot proliferation in Quercus subel L.Publication . Romano, A.; Noronha, C.; Martins-Loução, M. A.Procedures have been developed to standardize the multiplication stage during mature cork-oak (Quercus suber L.) micropropagation. Axillary and terminal buds were established on Gresshoff and Doy basal medium containing 1 mg l-1 of 6-benzylaminopurine (BAP). Initiation of cultures was possible all over the year. The effects of BAP, Z, IBA, IAA and NAA and various nutrient formulae on shoot growth and proliferation was investigated. BAP was more suitable than zeatin. Shoot proliferation and elongation were strongly improved by the combination BAP/IAA in the presence of low salt media, like Gresshoff and Doy or woody Plant medium. Both rates were significantly increased when a double-phase culture sytem was used. Shoots have been multiplied for 1 year at the rate of tree to four-fold every 4 weeks without any decline of vigour. Rooting was achieved by briefly dipping the basal ends of in vitro regenerated shoots in an IBA concentrated solution. The results here reported constitute a promising step towards large scale in vitro propagation of a species in which conventional vegetative propagation by cuttings is very difficult.
- Influence of sugars on in vitro rooting and acclimatization of carob treePublication . Custódio, L.; Martins-Loução, M. A.; Romano, A.Carob tree (Ceratonia siliqua L.) micropropagated shoots were rooted on half-strength Murashige and Skoog medium, supplemented with different types and concentrations of sugars, in order to determine the effects of sugar composition and concentration on in vitro rooting and in vivo establishment of the plantlets. Among the various sugars tested, the best rooting response was obtained with 145 mM sucrose, both in terms of rooting frequency and index of rooting. The use of filter-sterilized rather that autoclaved fructose increased root number and root length. Sugar treatment during rooting slightly influenced plantlet survival and growth during acclimatization. A reduction in the glucose concentration during rooting was beneficial for plantlet acclimatization.
- Micropropagation of mature cork-oak (Querqus suber L.) : establishment problemsPublication . Romano, A.; Martins-Loução, M. A.S’ han desenvolupat diversos mètodes per a l’ estandarizació de la fase d’ establiment durant la micropropagació de la surera (Quercus suber L.) adulta. Gemes axil.lars i terminals, cultivades, en medi de Gresshof y Doy (1972) (GD), van ser utilizades com a primer explant. L’establiment dels cultius va ser molt dificultós per causa de l’elevat ennegriment dels teixits y/o del medi i per l’elevada contaminació bacteriana. Els problems d’ennegriment, deguts probablement a l’exudació de compostos fenòlics, eren més importants a l’hivern. No obstant, es va aconsenguir l’establiment dels cultius all llarg de tot el cicle anyal, presumiblement pel precondicionament dels esqueixos. Els explants es van establir en medi GD adicionat de 1 mgl-1 de 6-benzilaminopurina (BAP). Cada 4 setmanes es procedia a efectuar un subcultiu en el mateix medi GD i a induir la proliferación amb una taxa de multiplicació de 4:1. S’ induia l’elongació dels brots mitjançant una disminució progressiva de la concentración de BAP. Els millors resultats per a l’ arrelament in vitro es van aconseguir sobre medi en agar solidificat adicionat amb 1 mgl-1 d’ àcid indolacètic (IAA). També es va assajar el medi líquid (sistema sorbarod) i l’arrelament in vivo. Procedures have been developed to standardize the establishment stage during mature cork-oak (Quercus suber L.) micropropagation. Axillary and terminal buds cultured in Gresshof and Doy (1972) (GD) medium were used as first explant. Establishment of cultures was very difficult due to browning of the tissue and/or the medium and bacterial contamination. Browning problems, probably due to phenolic compounds exudation of the primary explant, were found to be higher in winter. Nevertheless, initiation of cultures was possible all over the year, presumably due to the preconditioning of cuttings. Explants were established in a GD medium containing 6-benzlaminopurine (BAP) 1 mgl-1. Every 4 weeks the cultures were subcultured to the same GD medium and induced to proliferate being 4:1 the multiplication rate. Shoots were induced to elongate by decreasing BAP concentration. In vitro rooting on agar-solidified medium suplemented with 1 mgl-1 indolacetic acid (IAA) gave the best results. Liquid medium (sorbarod system) and in vivo rooting were also assayed.
- Micropropagation of the Mediterranean tree Ceratonia siliquaPublication . Romano, A.; Barros, S.; Martins-Loução, M. A.An in vitro propagation protocol based on axillary bud proliferation has been developed for mature female trees of Ceratonia siliqua L. ‘Galhosa’ and ‘Mulata’. Browning and contaminants were the major obstacles for culture establishment. Shoot culture initiation was greatly influenced by explanting season, with the highest survival percentage observed in spring. The cultivar, cytokinin type and concentration were the most important factors affecting shoot multiplication. The best multiple-shoot response was obtained with ‘Mulata’ on Murashige and Skoog medium supplemented with 4.44 μM6-benzyladenine or 4.56 μMzeatin. Rooting was achieved on growthregulator- free medium after basal dipping of shoots in indole-3-butyric acid (4.9 mM). Plantlets were successfully acclimatized (80 – 85%) under high relative humidity and then moved to the glasshouse. A field trial was established to follow their agronomic behaviour.
- Mycorrhization of cork oak micropropagated plantlets : its vitality during acclimatization measured with fast fluorescence techniquesPublication . Romano, A.; Strasser, R. J.; Eggenberg, P.; Martins-Loução, M. A.In this work we propose some fluorescense tests, based on a fast data acquisition (10 us per data point with 12 bit resolution) over five orders of magnitude in time (10 us to 1s) with an optoelectronic shutterless instrument (Plant Efficiency Analyser - PEA), to evaluate the response of micropropagated cork oak plantlets with or without mycorrhiza, in vitro and ex vitro conditions. Indexes have been defined which indicate the activity, efficiency, stress resistance, adaptability and developmental complexity of a plant. The shape of the observed fluorescence transient (on a logarithmic time scale) showed the typical Fo-J-I-P levels. The results obtained showed that mycorrhization increases the quantum yield of photochemistry as well as the electron transport and the photosynthetic metabolism. The density of photosynthetic units per leaf area is highly increased in mycorrhized plantlets. Therefore the productivity per leaf area is increased in these plantlets as well. During acclimatization mycorrhized plantlets show a much higher general fitness than non-mycorrhized plantlets in relation to the defined indexes.
- A new medium formulation for in vitro rooting of carob tree based on leaf macronutrients concentrationsPublication . Gonçalves, S.; Correia, P. J.; Martins-Loução, M. A.; Romano, A.Experiments were performed to optimize the macronutrients concentrations for in vitro rooting of Ceratonia siliqua micropropagated shoots. Several dilutions of Murashige and Skoog (MS) medium were tested: full-strength MS, halfstrength MS (½MS), and ½MS + full N. The frequency of in vitro rooting was enhanced when the ½MS was used (50 % rooted shoots). Mature leaves from 20 - 30 year-old carob trees and from 2 year-old micropropagated plants were collected and the concentrations of macronutrients (N, P, K, Ca, Mg) assessed. Based on the mineral composition of the leaves a new medium was formulated and compared with the previous ones showing an increment of the rooting frequency to 80 %. Moreover, shoots rooted in the new medium did not show symptoms of apical necrosis that occurred in the other tested media.
- Role of carbohydrates in micropropagation of cork oakPublication . Romano, A.; Noronha, C.; Martins-Loução, M. A.The influences of carbon sources, fructose, glucose, sorbitol and sucrose on shoot proliferation and in vitro rooting of cork oak (Quercus suber L.) were compared at a wide range of concentrations (1-6%, w/v). The highest number of shoots occurred on glucose-containing medium. Nevertheless, we have chosen 3% sucrose which induced a similar rate of proliferation but favoured shoot elongation, permitting an effectively higher number of shoots during transfers. Sorbitol and autoclaved fructose did not stimulate shoot proliferation. Adventitious root formation was strongly dependent on carbohydrate supply. Sorbitol and autoclaved fructose were completely ineffectively on rooting induction. Glucose was the most effective carbon source on rooting promotion followed by sucrose and filter-sterilized fructose. The rooting response induced by fructose was dependent on the sterilizing procedure. The number of adventitious roots produced per shoot increased with increasing glucose and sucrose concentration. The content of reducing sugars in leaves of proliferation cultures and in leaves and roots of rooted plantlets was more dependent on carbon concentration than on glucose or sucrose supplement. The results presented here show that carbohydrate requirements during cork oak micropropagation depend upon the phase of culture. Sucrose (3%) and glucose (4%) were the best carbon sources respectively during proliferation and rooting phases.