Browsing by Author "Carvalho, Ricardo Cruz"
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- Effects of Glyphosate-Based Herbicide on Primary Production and Physiological Fitness of the Macroalgae Ulva lactucaPublication . Carvalho, Ricardo Cruz; Feijão, Eduardo; Matos, Ana Rita; Cabrita, Maria Teresa; Utkin, Andrei B.The use of glyphosate-based herbicides (GBHs) worldwide has increased exponentially over the last two decades increasing the environmental risk to marine and coastal habitats. The present study investigated the effects of GBHs at environmentally relevant concentrations (0, 10, 50, 100, 250, and 500 g L1) on the physiology and biochemistry (photosynthesis, pigment, and lipid composition, antioxidative systems and energy balance) of Ulva lactuca, a cosmopolitan marine macroalgae species. Although GBHs cause deleterious effects such as the inhibition of photosynthetic activity, particularly at 250 g L1, due to the impairment of the electron transport in the chloroplasts, these changes are almost completely reverted at the highest concentration (500 g L1). This could be related to the induction of tolerance mechanisms at a certain threshold or tipping point. While no changes occurred in the energy balance, an increase in the pigment antheraxanthin is observed jointly with an increase in ascorbate peroxidase activity. These mechanisms might have contributed to protecting thylakoids against excess radiation and the increase in reactive oxygen species, associated with stress conditions, as no increase in lipid peroxidation products was observed. Furthermore, changes in the fatty acids profile, usually attributed to the induction of plant stress response mechanisms, demonstrated the high resilience of this macroalgae. Notably, the application of bio-optical tools in ecotoxicology, such as pulse amplitude modulated (PAM) fluorometry and laser-induced fluorescence (LIF), allowed separation of the control samples and those treated by GBHs in different concentrations with a high degree of accuracy, with PAM more accurate in identifying the different treatments
- Green roof design techniques to improve water use under Mediterranean conditionsPublication . Paço, Teresa Afonso do; Carvalho, Ricardo Cruz; Arsénio, Pedro; Martins, DianaGreen roof typology can vary depending on buildings structure, climate conditions, substrate, and plants used. In regions with hot and dry summers, such as the Mediterranean region, irrigation plays an essential role, as the highest temperatures occur during the driest period of the year. Irrigation might reduce the heat island effect and improve the cooling of buildings during this period, however, the added cost of maintenance operations and additional energy consumption could outrun the benefits provided by the project. Moreover, in situations where water is scarce or primarily channelled to other uses (e.g., domestic, agriculture or industry) during drought occurrence, it is advisable to implement green roof projects with the lowest use of water possible. The objective of the present work is to investigate solutions to optimize water use in green roofs under Mediterranean conditions, such as those of southern Europe. Two case studies are presented for Portugal, and potential techniques to reduce irrigation requirements in green roofs were tested. These addressed the use of native plant species, including the extreme type of a non-irrigated green roof (Biocrust roof) and techniques for plant installation. Plant drought tolerance was found to be an advantage in green roofs under these climatic conditions and, for the species studied, aesthetic value could be maintained when irrigation decreased
- Selecting potential moss species for green roofs in the Mediterranean BasinPublication . Carvalho, Ricardo Cruz; Varela, Zulema; Paço, Teresa Afonso do; Branquinho, CristinaGreen roofs are important infrastructures to address the e ects of climate change in urban areas. However, most studies and applications have been done in cooler and wetter regions of the northern hemisphere. Climate change will lead to more extreme weather events, such as increased drought and decreased precipitation with intense flash rain events. Increase desertification is expected especially in the Mediterranean Basin, where in summer, radiation and temperature are high and water is scarce. Therefore, while vascular plants increase water consumption in green roofs during warmer periods, mosses present themselves as potential candidates due to their poikilohydric nature, responding to the environmental availability of water, completely drying out and recovering upon rehydration. Although criteria for the selection of vascular plants adapted to the Mediterranean and suitable for green roofs have been developed, no information is available regarding the selection of mosses based on scientific criteria. Here we propose selection criteria for moss species based on ecological preferences according to Ellenberg’s values and help to define moss traits suitable for a nonirrigated, nature-based green roof that tolerates the Mediterranean climate. The main result is a table of potential candidate mosses that can be either used as standalone or in conjunction with vascular plants to decrease water usage and/or manage stormwater through an easily applicable selection methodology. For green roof practitioners, we proposed that acrocarpous mosses exhibiting turf/cushion life forms and colonist or perennial life strategies best fit the requirements for such a green infrastructure in extreme climate regions with scarce water resources
- Using chlorophyll a fluorescence imaging to select desiccation-tolerant native moss species for water-sustainable green roofsPublication . Carvalho, Ricardo Cruz; Paço, Teresa; Branquinho, Cristina; Silva, Jorge MarquesGreen roofs have been more thoroughly investigated in the last few years due to the potential benefits they o er to ecosystems in urban areas (e.g., carbon sequestration, particle retention, heat island e ect attenuation). However, current climate change models predict an increase in desertification, with an increase in temperature and decrease in rainfall, which means there is an increasing demand for green roofs with lower water consumption. Vegetation with very little water requirements, such as desiccation-tolerant mosses, has shown a potential to complement or substitute for vascular species, increasing the sustainability of lower water use in green roofs. In this study, we use chlorophyll a fluorescence imaging to screen for bryophytes with adequate physiology to be used in green roofs placed in at-risk areas with prolonged drought episodes. Apart from Hypnum cupressiforme, all selected species presented a high potential for use in those conditions, particularly Didymodon fallax, Grimmia lisae, Pleurochaete squarrosa, and Targionia hypophylla. Chlorophyll a fluorescence imaging technology proved to be a simple and non-invasive tool for a fast screening of these poikilohydric organisms, to be used in future studies of bryophyte biology, but more importantly in the green roof industry