Relatório integrador da actividade profissional

Abstract

This thesis constitutes a summary of my work for the past eight years. Given the different subjects that I have been involved, I selected three major lines of work to expose in this thesis, which are subdivided in four Chapters: Chapter I: “Descriptive Curriculum vitae” containing a brief presentation of the work developed, constituting a more descriptive cv, with a clear description of the projects I have been involved, techniques that I have become acquainted and a brief explanation of my responsibilities during this period. In Chapter II, entitled “Preliminary analysis of the antimicrobial protein content of the Spurge flax plant”, the antimicrobial content from the aqueous extract of the Spurge flax plant proved to be proteinaceous in nature, peptidic in size (<3 kDa) and resistant to denaturant treatments. In Chapter III, “Agrobacterium sp. mediated transformation of four Rosa hybrida cultivars with antifungal polypeptide BLAD”, the attempt towards the genetic modification of the rose plant with a BLAD potent antifungal polypeptide , is described. The final chapter, Chapter IV, “Optimization of the heterologous expression in E.coli of the antifungal polypeptide BLAD,” describes the different approaches applied towards the soluble over-expression in E.coli of the antifungal polypeptide BLAD.