Publicação
Resistência aos antimicrobianos em isolados de Salmonella spp. – Importância em Saúde Pública
| dc.contributor.advisor | Silveira,Leonor de Sousa Dâmaso da | |
| dc.contributor.advisor | Pina-Martins,Francisco | |
| dc.contributor.author | Caeiro,Raquel Fonseca | |
| dc.contributor.institution | Faculdade de Ciências | |
| dc.date.accessioned | 2026-05-21T10:40:03Z | |
| dc.date.available | 2026-05-21T10:40:03Z | |
| dc.date.issued | 2025 | |
| dc.description | Tese de mestrado, Biologia Humana e Ambiente, 2025, Universidade de Lisboa, Faculdade de Ciências | |
| dc.description.abstract | Salmonellosis is the second most common zoonosis in the European Union (EU), responsible for 77 486 infections in 2023. Due to the inappropriate use of antibiotics, the number of resistant Salmonella enterica isolates is increasing worldwide. In this study, isolates of ten serovars of Salmonella enterica enterica (N=310) from clinical samples received at INSA between January 1989 and June 2024 were characterized phenotypically and genotypically. During this period, there was a significant decrease in the number of resistant isolates in Portugal. With the phenotypic characterization, it was also observed that 16,8% (52/310) of the tested isolates were resistant, with the serovars S. Derby (52,6%; 20/38) and S. Saintpaul (50,0%; 9/18) presenting the highest percentage of resistant isolates. Of these resistant isolates, 36,5% (19/52) were multidrug resistant (MDR), the most frequent profile was AMP-TET-PEF (15,8%; 3/19) and S. Saintpaul was the serovar with the highest number of MDR isolates (88,9%; 8/9). The antibiotics with the highest number of resistances observed were tetracycline (13,5%; 41/310), sulfamethoxazole (8,7%; 27/310), pefloxacin (6,5%; 20/310) and ampicillin (5,5%; 17/310). Resistance to cephalosporins was also observed in lower percentages (between 0,5-1,0%). Whole genome sequencing (WGS) of the isolates allowed the identification of the genetic markers that conferred the resistant phenotypes, namely four genes encoding extended-spectrum β-lactamases (ESBLs) in isolates of S. Chester, S. Newport and S. Saintpaul. Genomic analysis also allowed the identification of two large clusters of S. Newport isolates and the identification of S. Bovismorbificans and S. Saintpaul isolates that were part of international clusters associated with foodborne outbreaks. This study highlights the importance of surveillance and monitoring of Salmonella spp. cases, so it is possible to exercise greater control over possible outbreaks and new resistance genetic markers, and the importance of the appropriate use of antimicrobial agents. | en |
| dc.format | application/pdf | |
| dc.identifier.tid | 204174120 | |
| dc.identifier.uri | http://hdl.handle.net/10400.5/118665 | |
| dc.language.iso | por | |
| dc.subject | Salmonella enterica | |
| dc.subject | Antimicrobial resistance | |
| dc.subject | WGS | |
| dc.subject | Surveillance | |
| dc.title | Resistência aos antimicrobianos em isolados de Salmonella spp. – Importância em Saúde Pública | pt |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | embargoedAccess |
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