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Espetroscopia de raios X na deteção de alterações elementares no esmalte dentário após branqueamento
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Objetivo: O objetivo deste estudo-piloto do tipo ensaio autocontrolado foi avaliar se existem alterações no conteúdo elementar do esmalte dentário quando é aplicada a técnica de branqueamento com peróxido de carbamida a 10%. Métodos: Utilizaram-se seis dentes anteriores higidos, extraídos por motivos ortodônticos ou periodontais e preservados em solução de cloramina 0,5% (w/w) num máximo de 6 meses. Foram realizados cortes dos dentes com recurso a um micrótomo de forma a obter amostras da face vestibular com 8 x 2 mm. As amostras foram então tratadas com o produto de branqueamento (Opalescence PF 10) conforme as instruções do fabricante e armazenadas em saliva artificial entre cada aplicação. Foi determinado o conteúdo elementar de cada amostra, antes e após o tratamento, por meio de micro energia dispersiva espectrometria de raios-x (μ-EDXRF). O equipamento consiste num tubo de raio x XTF5011 de OXFORD com um ânodo de Mo e um detector de silicio, com uma área ativa de 50 mm2 e uma espessura de janela de 12.5 μm. A radiação emitida pelo tubo de raios-x é focada por meio de uma lente policapilar, permitindo um ponto focal de 100 μm. A análise quantitativa das amostras foi realizada utilizando software WinAXIL. A análise estatística (teste t-student emparelhado) foi realizada com recurso ao SPSS v. 21. Os resultados de Ca e P são expressos em % (w/w) e o Zn em ppm (w/w). Resultados: As medições registadas após o branqueamento para Ca (31,49%), P (17,8%) e Zn (200,2 ppm) mostraram um decréscimo estatísticamente significativo (P<0.05) do conteúdo mineral quando comparado com os valores registados antes do tratamento Ca (32.81%), P (19,91%) e Zn (226,3 ppm). Conclusão: O procedimento de branqueamento realizado in vitro reduz o conteúdo mineral do esmalte. São necessários mais estudos que avaliem a significância clínica do presente estudo.
Objective: The purpose of this self-control pilot study was to assess whether the elemental content in tooth enamel is altered by a bleaching procedure the teeth with 10 % carbamide peroxide bleaching gel. Methods: Six anterior healthy teeth, extracted for periodontal or orthodontic reasons and preserved in a 0.5% (w/w) chloramine solution for no longer than 6 months were used. Sound vestibular surfaces of teeth were isolated. Cuts were made in order to obtain 8mm x 2mm samples. Samples were then treated with the bleaching product (Opalescence PF 10%) accordingly to manufacturer instructions and stocked in artificial inorganic saliva between each application. The elemental content of each samples, before and after treatment were determined by means of micro Energy Dispersive X-Ray Spectrometry (μ-EDXRF). The equipment consists on an X-ray tube OXFORD XTF5011 with a Mo anode and a Silicon Drift Detector Vortex-60EX® with an active area of 50mm2 and a 12.5μm thickness Be window. The radiation emitted by the X-ray tube is focused by means of polycapillary optics, allowing a focal spot of 100 μm. The quantitative analysis of the samples was carried out using WinAXIL software package. Statistical analysis (paired-sample t-Student Test) was performed by SPSS V. 21. Results of Ca and P are expressed in % (w/w) and Zn in ppm (w/w). Results: Measurements registered after bleaching for Ca (31,49%), P (17,8%) e Zn (200,2 ppm) showed a statistic significantly decrease (P<0.05) in mineral content when compared with values registered before treatment Ca (32.81%), P (19,91%) e Zn (226,3 ppm). Conclusion: The bleaching procedure performed in vitro reduced the mineral content of enamel. More studies are needed to assess the clinical significance of the present study.
Objective: The purpose of this self-control pilot study was to assess whether the elemental content in tooth enamel is altered by a bleaching procedure the teeth with 10 % carbamide peroxide bleaching gel. Methods: Six anterior healthy teeth, extracted for periodontal or orthodontic reasons and preserved in a 0.5% (w/w) chloramine solution for no longer than 6 months were used. Sound vestibular surfaces of teeth were isolated. Cuts were made in order to obtain 8mm x 2mm samples. Samples were then treated with the bleaching product (Opalescence PF 10%) accordingly to manufacturer instructions and stocked in artificial inorganic saliva between each application. The elemental content of each samples, before and after treatment were determined by means of micro Energy Dispersive X-Ray Spectrometry (μ-EDXRF). The equipment consists on an X-ray tube OXFORD XTF5011 with a Mo anode and a Silicon Drift Detector Vortex-60EX® with an active area of 50mm2 and a 12.5μm thickness Be window. The radiation emitted by the X-ray tube is focused by means of polycapillary optics, allowing a focal spot of 100 μm. The quantitative analysis of the samples was carried out using WinAXIL software package. Statistical analysis (paired-sample t-Student Test) was performed by SPSS V. 21. Results of Ca and P are expressed in % (w/w) and Zn in ppm (w/w). Results: Measurements registered after bleaching for Ca (31,49%), P (17,8%) e Zn (200,2 ppm) showed a statistic significantly decrease (P<0.05) in mineral content when compared with values registered before treatment Ca (32.81%), P (19,91%) e Zn (226,3 ppm). Conclusion: The bleaching procedure performed in vitro reduced the mineral content of enamel. More studies are needed to assess the clinical significance of the present study.
Descrição
Tese de Mestrado, Medicina Dentária, Universidade de Lisboa, Faculdade de Medicina Dentária, 2013
Palavras-chave
Dentisteria operatória Teses de mestrado - 2013