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Sequential dual site-selective protein labelling enabled by lysine modification

2020Journal article Restricted access
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dc.contributor.authorMatos, Maria J.
dc.contributor.authorBrown, Libby
dc.contributor.authorBernardim, Barbara
dc.contributor.authorGuerreiro, Ana
dc.contributor.authorJiménez-Osés, Gonzalo
dc.contributor.authorBernardes, Gonçalo J. L.
dc.date.accessioned2021-05-27T16:11:03Z
dc.date.available2021-05-27T16:11:03Z
dc.date.issued2020
dc.description© 2020 Elsevier Ltd. All rights reserved.pt_PT
dc.description.abstractMethods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.pt_PT
dc.description.sponsorshipWe thank funding from Xunta de Galicia (Galician Plan of research, innovation and growth 2011–2015, ED481B 2014/086-0 and ED481B 2018/007 to MJM), FAPESP (17/13168-8 to BB), FCT Portugal (FCT Investigator to GJLB, IF/00624/2015 and PhD studentship to AG, SFRH/BD/115932/2016), the Spanish Ministry of Science and Innovation (MCI) co-financed with FEDER funds (RTI2018-099592-B-C22 to G. J.O) and the BBSRC (iCASE studentship to LB). GJLB is a Royal Society University Research Fellow (URF\R\180019).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBioorg Med Chem. 2020 Nov 15;28(22):115783pt_PT
dc.identifier.doi10.1016/j.bmc.2020.115783pt_PT
dc.identifier.eissn1464-3391
dc.identifier.issn0968-0896
dc.identifier.urihttp://hdl.handle.net/10451/48213
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationIF/00624/2015pt_PT
dc.relationSynthetically-defined glycoconjugate vaccine candidates against Influenza
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/bioorganic-and-medicinal-chemistrypt_PT
dc.subjectAlbuminpt_PT
dc.subjectAntibodiespt_PT
dc.subjectBioorthogonal labellingpt_PT
dc.subjectLysine modificationpt_PT
dc.subjectProtein modificationpt_PT
dc.titleSequential dual site-selective protein labelling enabled by lysine modificationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSynthetically-defined glycoconjugate vaccine candidates against Influenza
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F115932%2F2016/PT
oaire.citation.issue22pt_PT
oaire.citation.titleBioorganic & Medicinal Chemistrypt_PT
oaire.citation.volume28pt_PT
oaire.fundingStreamOE
person.familyNamedos Santos Guerreiro
person.familyNameBernardes
person.givenNameAna Maria
person.givenNameGonçalo
person.identifier.ciencia-id481C-A494-B69D
person.identifier.orcid0000-0002-2173-6514
person.identifier.orcid0000-0001-6594-8917
person.identifier.scopus-author-id14046757500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1c4166db-cf53-48ab-9e3f-476f142cb304
relation.isAuthorOfPublicationd1a48067-77b1-4413-b1c7-602fb18c62c0
relation.isAuthorOfPublication.latestForDiscoveryd1a48067-77b1-4413-b1c7-602fb18c62c0
relation.isProjectOfPublication72b09169-6451-479e-b385-b00cd1aeec59
relation.isProjectOfPublication.latestForDiscovery72b09169-6451-479e-b385-b00cd1aeec59

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