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Publicação
Sequential dual site-selective protein labelling enabled by lysine modification
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Orientador(es)
Resumo(s)
Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.
Descrição
© 2020 Elsevier Ltd. All rights reserved.
Palavras-chave
Albumin Antibodies Bioorthogonal labelling Lysine modification Protein modification
Contexto Educativo
Citação
Bioorg Med Chem. 2020 Nov 15;28(22):115783
Editora
Elsevier