Sequential dual site-selective protein labelling enabled by lysine modification

Matos, Maria J.; Brown, Libby; Bernardim, Barbara; Guerreiro, Ana; Jiménez-Osés, Gonzalo; Bernardes, Gonçalo J. L.

http://hdl.handle.net/10451/48213

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Authors

Jiménez-Osés, Gonzalo

Bernardes, Gonçalo J. L.

Abstract(s)

Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.