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Transfection of pulmonary cells by stable pDNA-polycationic hybrid nanostructured particles

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dc.contributor.authorGaspar, Diana P.
dc.contributor.authorVital, Joana
dc.contributor.authorLeiva, María C.
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorTaboada, Pablo
dc.contributor.authorRemuñán-López, Carmen
dc.contributor.authorVítor, Jorge M. B.
dc.contributor.authorAlmeida, António José
dc.date.accessioned2023-09-14T13:54:37Z
dc.date.available2023-09-14T13:54:37Z
dc.date.issued2019
dc.date.updated2023-02-27T18:53:38Z
dc.description© 2019 Future Medicine Ltdpt_PT
dc.description.abstractAim: Cationically modified solid lipid nanoparticles (SLN) were investigated as plasmid DNA (pDNA) carriers and transfection agents for the pulmonary route. Materials & methods:pDNA-loaded SLN were produced using glyceryl dibehenate or tristearate as matrix lipids and chitosan as surface charge modifier, and encapsulated by spray-drying in mannitol and trehalose microspheres. Results: Nanoparticles of 200 nm, and zeta potential around +15 mV were produced. Electrophorectic analysis confirmed plasmid stability and integrity. The pDNA-loaded SLN were able to transfect the Calu-3 and A549 pulmonary cell lines, while showing low cytotoxicity. Microencapsulation of SLN yielded dry powders suitable for inhalation that protected pDNA from degradation. Conclusion: Microencapsulated SLN are a promising safe and effective carrier system for pulmonary gene delivery following pulmonary administration.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e Tecnologia, Portugal (projects UID/DTP/04138/2013 and PTDC/DTPFTO/0094/2012, and grants SFRH/BD/89520/2012 and SFRH/BSAB/1210/2011). This work was also supported by Spanish Government (PN de I+D 2008-2011, ISCIII-Subdireccion General de Evaluación y Fomento de la Investigación, Acción Estratégica de Salud, Projects FIS, PS09/00816, and MAT2013-40971-R), Xunta de Galicia (Project Competitive Reference Groups-FEDER Funds Ref. 2014/043 and Project Competitive Emergent Groups-FEDER Funds EM2013-046). Jorge Vitor’s lab has been financed by New England Biolabs, Inc. (USA). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGaspar DP, Vital J, Leiva MC, Gonçalves LM, Taboada P, Remuñán-López C, et al. Transfection of pulmonary cells by stable pDNA -polycationic hybrid nanostructured particles. Nanomedicine [Internet]. fevereiro de 2019;14(4):407–29. Disponível em: https://www.futuremedicine.com/doi/10.2217/nnm-2018-0270pt_PT
dc.identifier.doi10.2217/nnm-2018-0270pt_PT
dc.identifier.eid2-s2.0-85061585165
dc.identifier.slugcv-prod-2420944
dc.identifier.urihttp://hdl.handle.net/10451/59295
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFuture Medicinept_PT
dc.relationUID/DTP/04138/2013pt_PT
dc.relationPTDC/DTPFTO/0094/2012pt_PT
dc.relationSFRH/BD/89520/2012pt_PT
dc.relationSFRH/BSAB/1210/2011pt_PT
dc.relation.publisherversionhttps://www.futuremedicine.com/doi/epub/10.2217/nnm-2018-0270pt_PT
dc.subjectchitosanpt_PT
dc.subjectgene deliverypt_PT
dc.subjectinhalationpt_PT
dc.subjectmicroencapsulationpt_PT
dc.subjectplasmidpt_PT
dc.subjectsolid lipid nanoparticlespt_PT
dc.subjectspray-dryingpt_PT
dc.titleTransfection of pulmonary cells by stable pDNA-polycationic hybrid nanostructured particlespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage429pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage407pt_PT
oaire.citation.titleNanomedicinept_PT
oaire.citation.volume14pt_PT
person.familyNameDiogo Gonçalves
person.familyNameVítor
person.familyNameLeitão das Neves Almeida
person.givenNameLídia Maria
person.givenNameJorge
person.givenNameAntónio José
person.identifierI-6590-2012
person.identifier.ciencia-id7211-22BA-86AD
person.identifier.ciencia-idFF19-99F4-3964
person.identifier.ciencia-id8312-F853-C47A
person.identifier.orcid0000-0002-6799-2740
person.identifier.orcid0000-0001-6486-3444
person.identifier.orcid0000-0002-7807-4726
person.identifier.ridM-3279-2013
person.identifier.scopus-author-id7801493621
person.identifier.scopus-author-id57195052432
rcaap.cv.cienciaid7211-22BA-86AD | Lídia Maria Diogo Gonçalves
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3265148d-dc2d-4601-a832-2e72f7be5662
relation.isAuthorOfPublicationbe15e90f-9cc9-4ad0-b9fd-465236b72e9a
relation.isAuthorOfPublicationdab457ee-7a1d-4c3d-a906-1e8df92fffd6
relation.isAuthorOfPublication.latestForDiscovery3265148d-dc2d-4601-a832-2e72f7be5662

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