A carregar...
Publicação
Transfection of pulmonary cells by stable pDNA-polycationic hybrid nanostructured particles
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 19.53 MB | Adobe PDF |
Orientador(es)
Resumo(s)
Aim: Cationically modified solid lipid nanoparticles (SLN) were investigated as plasmid DNA (pDNA) carriers and transfection agents for the pulmonary route. Materials & methods:pDNA-loaded SLN were produced using glyceryl dibehenate or tristearate as matrix lipids and chitosan as surface charge modifier, and encapsulated by spray-drying in mannitol and trehalose microspheres. Results: Nanoparticles of 200 nm, and zeta potential around +15 mV were produced. Electrophorectic analysis confirmed plasmid stability and integrity. The pDNA-loaded SLN were able to transfect the Calu-3 and A549 pulmonary cell lines, while showing low cytotoxicity. Microencapsulation of SLN yielded dry powders suitable for inhalation that protected pDNA from degradation. Conclusion: Microencapsulated SLN are a promising safe and effective carrier system for pulmonary gene delivery following pulmonary administration.
Descrição
© 2019 Future Medicine Ltd
Palavras-chave
chitosan gene delivery inhalation microencapsulation plasmid solid lipid nanoparticles spray-drying
Contexto Educativo
Citação
Gaspar DP, Vital J, Leiva MC, Gonçalves LM, Taboada P, Remuñán-López C, et al. Transfection of pulmonary cells by stable pDNA -polycationic hybrid nanostructured particles. Nanomedicine [Internet]. fevereiro de 2019;14(4):407–29. Disponível em: https://www.futuremedicine.com/doi/10.2217/nnm-2018-0270
Editora
Future Medicine