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A colorimetric process to visualize erythrocyte exovesicles aggregates

2004Artigo científico Acesso restrito
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dc.contributor.authorSaldanha, Carlota
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorMartins e Silva, João
dc.date.accessioned2022-03-11T16:57:31Z
dc.date.available2022-03-11T16:57:31Z
dc.date.issued2004
dc.description© 2004 by The International Union of Biochemistry and Molecular Biologypt_PT
dc.description.abstractA biochemistry laboratory class protocol is described in order to create an opportunity for students to apply by doing the theoretical concepts underlying biomolecules and vesicles properties, together with the principles of centrifugation and colorimetric methodologies. Through simple procedures the students will i) observe the segregation of the vesicles suspensions into two separate phases (phtalate esters gradient and vesicle aggregates); ii) visualize the vesicle aggregates by protein, enzyme, and phospholipids coloration processes; and iii) discuss and explain the visualized colors by proper biochemical reactions. The aims, objectives, methodology of teaching/learning, and assessment for this laboratory class are indicated.pt_PT
dc.description.abstract, ,pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBiochem Mol Biol Educ. 2004 Jul;32(4):250-253pt_PT
dc.identifier.doi10.1002/bmb.2004.494032040374pt_PT
dc.identifier.eissn1539-3429
dc.identifier.issn470-8175
dc.identifier.urihttp://hdl.handle.net/10451/51706
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://iubmb.onlinelibrary.wiley.com/journal/15393429pt_PT
dc.subjectExovesiclept_PT
dc.subjectErythrocytept_PT
dc.subjectAcetylcholinesterasept_PT
dc.subjectMembranept_PT
dc.titleA colorimetric process to visualize erythrocyte exovesicles aggregatespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage253pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage250pt_PT
oaire.citation.titleBiochemistry and Molecular Biology Educationpt_PT
oaire.citation.volume32pt_PT
person.familyNameSaldanha
person.familyNameSantos
person.familyNamemartins e silva
person.givenNameCarlota
person.givenNameNuno
person.givenNamejoao
person.identifier53901
person.identifier.ciencia-idCD13-5E3A-A3C5
person.identifier.orcid0000-0002-5058-2112
person.identifier.orcid0000-0002-0580-0475
person.identifier.orcid0000-0002-1382-715X
person.identifier.ridN-7248-2013
person.identifier.scopus-author-id55940818300
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationad6de30c-87ca-4613-bb87-488dd5d285d0
relation.isAuthorOfPublication4656d912-5a2d-4c80-8921-2bcba3aa441a
relation.isAuthorOfPublication5f6b25d7-bbd9-4abf-aed5-966190524c83
relation.isAuthorOfPublication.latestForDiscovery4656d912-5a2d-4c80-8921-2bcba3aa441a

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