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A colorimetric process to visualize erythrocyte exovesicles aggregates
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Orientador(es)
Resumo(s)
A biochemistry laboratory class protocol is described in order to create an opportunity for students to apply by doing the theoretical concepts underlying biomolecules and vesicles properties, together with the principles of centrifugation and colorimetric methodologies. Through simple procedures the students will i) observe the segregation of the vesicles suspensions into two separate phases (phtalate esters gradient and vesicle aggregates); ii) visualize the vesicle aggregates by protein, enzyme, and phospholipids coloration processes; and iii) discuss and explain the visualized colors by proper biochemical reactions. The aims, objectives, methodology of teaching/learning, and assessment for this laboratory class are indicated.
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Descrição
© 2004 by The International Union of Biochemistry and Molecular Biology
Palavras-chave
Exovesicle Erythrocyte Acetylcholinesterase Membrane
Contexto Educativo
Citação
Biochem Mol Biol Educ. 2004 Jul;32(4):250-253
Editora
Wiley