Loading...
Publication
Estudo parasitário de lobos, cães e raposas na margem sul do Douro, Portugal continental
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 2.41 MB | Adobe PDF |
Abstract(s)
A interação entre canídeos selvagens e domésticos, como lobo-ibérico (Canis lupus signatus), raposa-vermelha (Vulpes vulpes silacea) e cão doméstico (Canis lupus familiaris), em ambientes compartilhados facilita a transmissão de parasitas gastrointestinais, tornando essencial o estudo desses parasitas nestas populações. Esta dissertação pretende preencher a lacuna de pesquisas parasitológicas na região ao sul do Douro, concentrando-se nos parasitas gastrointestinais que afetam as três espécies de canídeos nessa área. Foram colhidas 85 amostras de fezes de canídeos da margem sul do Douro entre setembro e dezembro de 2022, e analisadas no Laboratório de Parasitologia e Doenças Parasitárias da FMV usando técnicas coprológicas, incluindo flutuação, sedimentação e 3 repetições de McMaster por amostra. Os dados, que incluíram identificação, espécie, coordenadas geográficas e resultados coprológicos, foram inicialmente inseridos no software Epicollect5, transferidos para Microsoft Excel 2016 e exportados para IBM SPSS 29.0.2.0. As análises estatísticas, realizadas com um nível de significância de p<0,05, incluíram estatística descritiva, ANOVA, testes de Qui-quadrado, testes de Fisher e esfericidade de Mauchly. Das 85 amostras recolhidas, sendo 60 de raposa (70,6%), 16 de cão (18,8%) e 9 de lobo (10,6%), 60% (51/85) estavam parasitadas. Obtivemos uma prevalência de 76% (47/51) de Ancylostoma sp., 18% (11/51) de Toxascaris leonina e 6% (4/51) de Toxocara canis. A maioria das amostras parasitadas (78,4%, 40/51) apresentaram infeções simples, enquanto 21,6% (11/51) exibiram infeções mistas. A combinação mais comum nas infeções mistas foi entre Toxocara canis e Ancylostoma sp. (64%, 7/11) seguida pela combinação de Toxascaris leonina e Ancylostoma sp. (36%, 4/11). Não foram observadas coinfecções entre Toxascaris leonina e Toxocara canis. O teste de McNemar revelou uma diferença significativa na deteção de Ancylostoma sp. entre os métodos de flutuação e sedimentação (p<0,001). No entanto, ambos os métodos mostraram desempenho comparável na identificação de Toxascaris leonina e Toxocara canis, sem diferenças significativas (p=0,250 e p=0,500, respetivamente). Este trabalho é importante para aprofundar o conhecimento sobre a interação entre parasitas de canídeos domésticos e silvestres na margem sul do Douro, destacando a necessidade de monitorização contínua e estratégias de desparasitação eficazes para proteger tanto a fauna silvestre, quanto a saúde pública na região
ABSTRACT - PARASITOLOGICAL STUDY OF WOLVES, DOGS AND FOXES ON THE SOUTH BANK OF THE DOURO, MAINLAND PORTUGAL - The interaction between wild and domestic canids, such as the Iberian wolf (Canis lupus signatus), red fox (Vulpes vulpes silacea), and domestic dog (Canis lupus familiaris), in shared environments, facilitates the transmission of gastrointestinal parasites, making essential their study in these populations. This dissertation aims to address the lack of parasitological research in the region south of the Douro, focusing on the gastrointestinal parasites affecting these three species of canids in this area. Eighty-five faecal samples from canids in the southern Douro region were collected between September and December 2022 and analysed at the Laboratory of Parasitology and Parasitic Diseases of FMV using coprological techniques, including flotation, sedimentation, and 3 repetitions of McMaster per sample. The data, including identification, species, geographical coordinates, and coprological results, were initially entered into the Epicollect5 software, transferred to Microsoft Excel 2016, and exported to IBM SPSS 29.0.2.0. Statistical analyses, conducted with a significance level of p<0.05, included descriptive statistics, ANOVA, Chi-square tests, Fisher’s exact tests and Mauchly’s test of sphericity. Of the 85 samples collected - 60 from foxes (70.6%), 16 from dogs (18.8%), and 9 from wolves (10,6%) - 60% (51/85) were parasitised. We detected 76% (47/51) of Ancylostoma sp., 18% (11/51) of Toxascaris leonina, and 6% (4/51) of Toxocara canis. Most of the parasitised samples (78,4%, 40/51) had single infections, while 21.6% (11/51) had mixed infections. The most common combination in mixed infections was between Toxocara canis and Ancylostoma sp. (64%, 7/11), followed by the combination of Toxascaris leonina and Ancylostoma sp. (36%, 4/11). No coinfections were observed between Toxascaris leonina and Toxocara canis. The McNemar test revealed a significant difference in the detection of Ancylostoma sp. between the flotation and sedimentation methods (p<0.001). However, both methods showed comparable performance in identifying Toxascaris leonina and Toxocara canis, with no significant differences (p=0.250 and p=0.500, respectively). This work is important for deepening the understanding of the interaction between parasites of domestic and wild canids in the southern Douro region, highlighting the need for continuous monitoring and effective deworming strategies to protect both wildlife and public health in the area
ABSTRACT - PARASITOLOGICAL STUDY OF WOLVES, DOGS AND FOXES ON THE SOUTH BANK OF THE DOURO, MAINLAND PORTUGAL - The interaction between wild and domestic canids, such as the Iberian wolf (Canis lupus signatus), red fox (Vulpes vulpes silacea), and domestic dog (Canis lupus familiaris), in shared environments, facilitates the transmission of gastrointestinal parasites, making essential their study in these populations. This dissertation aims to address the lack of parasitological research in the region south of the Douro, focusing on the gastrointestinal parasites affecting these three species of canids in this area. Eighty-five faecal samples from canids in the southern Douro region were collected between September and December 2022 and analysed at the Laboratory of Parasitology and Parasitic Diseases of FMV using coprological techniques, including flotation, sedimentation, and 3 repetitions of McMaster per sample. The data, including identification, species, geographical coordinates, and coprological results, were initially entered into the Epicollect5 software, transferred to Microsoft Excel 2016, and exported to IBM SPSS 29.0.2.0. Statistical analyses, conducted with a significance level of p<0.05, included descriptive statistics, ANOVA, Chi-square tests, Fisher’s exact tests and Mauchly’s test of sphericity. Of the 85 samples collected - 60 from foxes (70.6%), 16 from dogs (18.8%), and 9 from wolves (10,6%) - 60% (51/85) were parasitised. We detected 76% (47/51) of Ancylostoma sp., 18% (11/51) of Toxascaris leonina, and 6% (4/51) of Toxocara canis. Most of the parasitised samples (78,4%, 40/51) had single infections, while 21.6% (11/51) had mixed infections. The most common combination in mixed infections was between Toxocara canis and Ancylostoma sp. (64%, 7/11), followed by the combination of Toxascaris leonina and Ancylostoma sp. (36%, 4/11). No coinfections were observed between Toxascaris leonina and Toxocara canis. The McNemar test revealed a significant difference in the detection of Ancylostoma sp. between the flotation and sedimentation methods (p<0.001). However, both methods showed comparable performance in identifying Toxascaris leonina and Toxocara canis, with no significant differences (p=0.250 and p=0.500, respectively). This work is important for deepening the understanding of the interaction between parasites of domestic and wild canids in the southern Douro region, highlighting the need for continuous monitoring and effective deworming strategies to protect both wildlife and public health in the area
Description
Dissertação de Mestrado Integrado em Medicina Veterinária, área científica de Sanidade Animal
Keywords
Canídeos Coprologia Parasitas gastrointestinais Rio Douro Portugal Canids Coprology Gastrointestinal parasites River Douro Portugal
Pedagogical Context
Citation
Oliveira FSGS. 2025. Estudo parasitário de lobos, cães e raposas na margem sul do Douro, Portugal continental [dissertação de mestrado]. Lisboa: FMV-Universidade de Lisboa
Publisher
Universidade de Lisboa, Faculdade de Medicina Veterinária