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Colagénio, eosinófilos e interleucina 33 no endométrio da burra
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Os eosinófilos e a fibrose no endométrio da burra podem influenciar a função reprodutiva. A classificação histopatológica do endométrio da égua de Kenney e Doig é usada na burra. A interação da IL-33 e dos eosinófilos tem sido associada à fibrose. Este estudo no endométrio de burra classificado segundo Kenney e Doig teve como objetivos: (i) quantificar a transcrição dos genes de colagénio tipo I (COL1), colagénio tipo III (COL3) e da interleucina 33 (IL-33) e sua correlação com o número de eosinófilos; bem como (ii) localizar e quantificar microscopicamente o COL1 e COL3. O número de eosinófilos e neutrófilos diminuiu com a fibrose (P < 0,05). Os transcritos de IL-33 tenderam a aumentar da categoria IIA para III. Houve uma tendência para uma correlação positiva entre o número de eosinófilos e transcritos de IL-33 na categoria IIA (ρ = 0,7714; P = 0,055). A área de COL1 e COL3 e o número de neutrófilos apresentaram uma correlação positiva e negativa, respetivamente. O facto do COL3 se localizar maioritariamente sob o epitélio de superfície e extrato compacto em todas as categorias de endométrio parece não ter um efeito nocivo na implantação embrionária e explicar a maior longevidade reprodutiva nesta espécie animal.
ABSTRACT - COLLAGEN, EOSINOPHILS AND INTERLEUKIN 33 IN JENNY´S ENDOMETRIUM - Eosinophils and fibrosis in the endometrium of the jennies may influence their reproductive function. The Kenney and Doig histopatological classification of the endometrium developed for the mare is used in jennies. The interaction between IL-33 and eosinophils has been associated with fibrosis. This study on the jenny endometrium classified according to Kenney and Doig had the following objectives: (i) quantification of collagen type I (COL1), collagen type III (COL3), and interleukin 33 (IL-33) transcripts and their correlation with the number of eosinophils; as well as (ii) the histological localization and quantification of COL1 and COL3. The number of eosinophils and neutrophils decreased with the fibrosis (P < 0.05). IL-33 transcripts increased from category IIA to III. There was a tendency towards a positive correlation between eosinophil count and IL-33 transcripts in category IIA (ρ = 0,7714; P = 0,055). The area of COL1 and COL3 and the number of neutrophils showed a positive and negative correlation, respectively. The presence of COL3 located mostly under the surface epithelium and stratum compactum in all endometrial categories may not have a deleterious effect on embryo implantation and might explain the reproductive longevity in this animal species.
ABSTRACT - COLLAGEN, EOSINOPHILS AND INTERLEUKIN 33 IN JENNY´S ENDOMETRIUM - Eosinophils and fibrosis in the endometrium of the jennies may influence their reproductive function. The Kenney and Doig histopatological classification of the endometrium developed for the mare is used in jennies. The interaction between IL-33 and eosinophils has been associated with fibrosis. This study on the jenny endometrium classified according to Kenney and Doig had the following objectives: (i) quantification of collagen type I (COL1), collagen type III (COL3), and interleukin 33 (IL-33) transcripts and their correlation with the number of eosinophils; as well as (ii) the histological localization and quantification of COL1 and COL3. The number of eosinophils and neutrophils decreased with the fibrosis (P < 0.05). IL-33 transcripts increased from category IIA to III. There was a tendency towards a positive correlation between eosinophil count and IL-33 transcripts in category IIA (ρ = 0,7714; P = 0,055). The area of COL1 and COL3 and the number of neutrophils showed a positive and negative correlation, respectively. The presence of COL3 located mostly under the surface epithelium and stratum compactum in all endometrial categories may not have a deleterious effect on embryo implantation and might explain the reproductive longevity in this animal species.
Descrição
Dissertação de Mestrado Integrado em Medicina Veterinária
Palavras-chave
Burro Endométrio Endometrose Colagénio Eosinófilo IL-33 Donkey Endometrium Endometrosis Collagen Eosinophils IL-33
Contexto Educativo
Citação
Silva JAN. 2022. Colagénio, eosinófilos e interleucina 33 no endométrio da burra [dissertação de mestrado]. Lisboa: FMV-Universidade de Lisboa
Editora
Universidade de Lisboa, Faculdade de Medicina Veterinária