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Orientador(es)
Resumo(s)
In root tip cells from seedlings of Secale cereale exposed to high temperatures (heat stress),
marked modifications in nuclear morphology and microtubule cytoskeleton dynamics are
observed. Seedlings were developed in normal environmental conditions at 21ºC, and
transferred for 40ºC during four hours for the heat shock treatment. Root-tips were excised
just after the treatment, and after distinct periods of recovery (RT). The detailed
characterization of meristematic cells nuclear morphology revealed striking modifications
some of which are maintained even after 24 of RT. These alterations seem to be associated
with changes in cell cycle. Imunocytochemistry was used to pursue the study of microtubules
(MTs) dynamics in heat treated plants using alfa-tubulin antibodies. These revealed great
alterations in MTs organization, preferentially affecting interphase cells. These results clearly
indicate for the first time a direct impact of heat stress on nuclear dynamics and cell cycle
progression
Descrição
Relatório da Bolsa de integração na Investigação (BII)
Palavras-chave
Secale cereale heat stress chromatin cytoskeleton
Contexto Educativo
Citação
Neves, V.R.A. - Análise do efeito das altas temperaturas na organização da cromatina e do citoesqueleto microtubular em células da raiz de Secale cereale. Lisboa: CBAA/ISA, 2009