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Phellem cell-wall components are discriminants of cork quality in Quercus suber
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Orientador(es)
Resumo(s)
Cork is a renewable, non-wood high valued forest product, with relevant ecological
and economic impact in the Mediterranean-type ecosystems. Currently, cork is ranked
according to its commercial quality. The most valuable planks are chosen for cork
stoppers production. Cork planks with adequate thickness and porosity are classified
as stoppable quality cork (SQC). The chemical composition of cork is known, but
the regulation of metabolic pathways responsible of cork production and composition,
hence of cork quality, is largely unknown. Here, we tested the hypothesis that
post-genomic events may be responsible for the development of SQC and N-SQC
(non-stoppable quality cork). Here, we show that combined proteomics and targeted
metabolomics (namely soluble and cell wall bound phenolics) analyzed on recently
formed phellem allows discriminate cork planks of different quality. Phellem cells of
SQC and N-SQC displayed different reducing capacity, with consequential impact
on both enzymatic pathways (e.g., glycolysis) and other cellular functions, including
cell wall assembly and suberization. Glycolysis and respiration related proteins were
abundant in both cork quality groups, whereas the level of several proteins associated
to mitochondrial metabolism was higher in N-SQC. The soluble and cell wall-bound
phenolics in recently formed phellem clearly discriminated SQC from N-SCQ. In our
study, SQC was characterized by a high incorporation of aromatic components of the
phenylpropanoid pathway in the cell wall, together with a lower content of hydrolysable
tannins. Here, we propose that the level of hydrolysable tannins may represent a valuable
diagnostic tool for screening recently formed phellem, and used as a proxy for the quality
grade of cork plank produced by each tree
Descrição
Original Research
Palavras-chave
hydrolysable tannins proteomics targeted metabolomics soluble phenolics cell-wall immobilized phenolics Quercus suber
Contexto Educativo
Citação
Pinheiro C, Wienkoop S, de Almeida JF, Brunetti C, Zarrouk O, Planchon S, Gori A, Tattini M, Ricardo CP, Renaut J and Teixeira RT (2019) Phellem Cell-Wall Components Are Discriminants of Cork Quality in Quercus suber. Front. Plant Sci. 10:944
Editora
Soren K. Rasmussen, University of Copenhagen, Denmark