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Authors
Abstract(s)
Cryptosporidium spp. e Giardia duodenalis são agentes comuns de doença gastrointestinal, quer em humanos, quer em animais. A principal via de transmissão é a fecal oral através da ingestão de águas ou alimentos contaminados com oocistos de Cryptosporidium spp. ou de quistos de Giardia duodenalis, ou através de contacto direto com animais infetados. Ambos os parasitas representam um problema sério tanto na saúde animal como em saúde pública, uma vez que algumas espécies e genótipos são zoonóticos. O hospedeiro infetado apresenta frequentemente alterações gastrointestinais, como vómitos e diarreias associadas a perda de peso. O objetivo deste estudo foi estudar a prevalência de Cryptosporidium spp. e Giardia duodenalis no intestino de morcegos provenientes de São Paulo, no Brasil, através de técnicas de biologia molecular. Para isso foram testadas 190 amostras de intestino de morcegos, 96 hematófagos e 94 não hematófagos, para a presença de DNA de Cryptosporidium spp. e de Giardia duodenalis através de reações de PCR tendo como alvo o gene SSU rRNA. A prevalência de Cryptosporidium spp. e Giardia duodenalis em morcegos provenientes do estado de São Paulo, Brasil, foi de 0% para ambos os parasitas testados com os primers utilizados. Tendo em conta a importância de Cryptosporidium spp. e de Giardia duodenalis para a saúde pública e veterinária e estando estes parasitas frequentemente presentes em alimentos e águas, apesar de não terem sido detetados em nenhuma das amostras analisadas neste estudo, não significa que não possam estar presentes nas populações de morcegos de São Paulo, justificando por isso a necessidade de estudos futuros dada a importância destes dois protozoários no contexto de uma só saúde
ABSTRACT - Detection of Cryptosporidum spp. and Giardia duodenalis DNA in bats from Brazil - Cryptosporidium spp. and Giardia duodenalis are common agents of gastrointestinal disease in both humans and animals. The main route of transmission is fecal-oral through the ingestion of water or food contaminated with Cryptosporidium spp. oocysts or Giardia duodenalis cysts, or through direct contact with infected animals. Both parasites represent a serious problem for both animal and public health, since some species and genotypes are zoonotic. The infected host often presents gastrointestinal alterations, such as vomiting and diarrhea associated with weight loss. The aim of this study was to study the prevalence of Cryptosporidium spp. and Giardia duodenalis in the intestines of bats from São Paulo, Brazil, using molecular biology techniques. To this end, 190 bat intestinal samples, 96 hematophagous and 94 non-hematophagous, were tested for the presence of Cryptosporidium spp. and Giardia duodenalis DNA using PCR reactions targeting the SSU rRNA gene. The prevalence of Cryptosporidium spp. and Giardia duodenalis in bats from the state of São Paulo, Brazil, was 0% for both parasites tested with the primers used. Given the importance of Cryptosporidium spp. and Giardia duodenalis for public and veterinary health and the fact that these parasites are often present in food and water, although they were not detected in any of the samples analyzed in this study, this does not mean that they cannot be present in the bat populations of São Paulo. This justifies the need for future studies given the importance of these two protozoa in the context of a single health
ABSTRACT - Detection of Cryptosporidum spp. and Giardia duodenalis DNA in bats from Brazil - Cryptosporidium spp. and Giardia duodenalis are common agents of gastrointestinal disease in both humans and animals. The main route of transmission is fecal-oral through the ingestion of water or food contaminated with Cryptosporidium spp. oocysts or Giardia duodenalis cysts, or through direct contact with infected animals. Both parasites represent a serious problem for both animal and public health, since some species and genotypes are zoonotic. The infected host often presents gastrointestinal alterations, such as vomiting and diarrhea associated with weight loss. The aim of this study was to study the prevalence of Cryptosporidium spp. and Giardia duodenalis in the intestines of bats from São Paulo, Brazil, using molecular biology techniques. To this end, 190 bat intestinal samples, 96 hematophagous and 94 non-hematophagous, were tested for the presence of Cryptosporidium spp. and Giardia duodenalis DNA using PCR reactions targeting the SSU rRNA gene. The prevalence of Cryptosporidium spp. and Giardia duodenalis in bats from the state of São Paulo, Brazil, was 0% for both parasites tested with the primers used. Given the importance of Cryptosporidium spp. and Giardia duodenalis for public and veterinary health and the fact that these parasites are often present in food and water, although they were not detected in any of the samples analyzed in this study, this does not mean that they cannot be present in the bat populations of São Paulo. This justifies the need for future studies given the importance of these two protozoa in the context of a single health
Description
Dissertação de Mestrado Integrado em Medicina Veterinária, área científica de Sanidade Animal
Keywords
Cryptosporidium spp. Giardia duodenalis PCR Morcegos Brasil Cryptosporidium spp. Giardia duodenalis PCR Bats Brazil
Pedagogical Context
Citation
Andrade PS. 2024. Deteção de DNA de Cryptosporidium spp. e de Giardia duodenalis em morcegos provenientes do Brasil [dissertação de mestrado]. Lisboa: FMV-Universidade de Lisboa
Publisher
Universidade de Lisboa, Faculdade de Medicina Veterinária