A carregar...
Publicação
Erosão dentária: estudo in-vitro
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 8.53 MB | Adobe PDF |
Orientador(es)
Resumo(s)
A erosão dentária é um processo crónico, indolor com perda irreversível de tecido dentário devido a um processo químico que não envolve microorganismos. Objectivo: Este estudo in vitro pretende avaliar os efeitos de erosão efectuados pela Coca Cola, Só Laranja e Red Bull, na superfície de amostras de esmalte humano através da observação ao microscópio electrónico de varrimento (SEM). Materiais e métodos: O pH de cada bebida foi determinado com recurso a um potenciómetro de pH. A capacidade tampão foi determinada após a adição repetida de 0,025 ml de NaOH a 2% em água, em 8 ml de solução de cada bebida até atingir pH de 7. A coroa de cada um dos quatro molares humanos foi seccionada da raiz e submetida a um corte no sentido mesio-distal. Foi desenhada uma janela de exposição de esmalte rectangular nas faces vestibular e lingual. Utilizando a faca Isomet 1000 obtiveram-se duas janelas de exposição rectangulares, que foram cortadas ao meio para se obterem na totalidade dezasseis espécimes. A área externa à janela de exposição foi coberta com verniz. Os espécimes pintados com a mesma cor de verniz foram reunidos e formaramse quatro grupos. Foi assegurada a inclusão de um espécime correspondente a cada molar em cada grupo. Aleatoriamente, três grupos foram sujeitos a quatro ciclos de desmineralização/remineralização e o quarto grupo continuou a ser imergido em saliva artificial. No final do procedimento experimental, foram retirados e preparados quatro espécimes de cada grupo para análise de superficíe ao SEM. Resultados: A bebida Coca-Cola apresenta pH inicial mais baixo (pH=2,54) e a bebida Red Bull possui maior capacidade tampão. As bebidas acídicas seleccionadas promoveram desgaste ao nível da superfície de esmalte humano e a saliva artificial não causou alterações no esmalte. Conclusão: O potencial erosivo das bebidas ácidas seleccionadas foi confirmado no presente estudo.
Dental erosion is a chronic process, painless with irreversible loss of tooth tissue due to a chemical process that does not involve microorganisms. Objective: This in vitro study intends to evaluate the effects of erosion made by Coca Cola, Só Laranja and Red Bull, on the surface of samples of human enamel through the observation on the scanning electron microscope (SEM). Materials and methods: The pH of each beverage was determined using a potentiometer pH. The buffer capacity was determined after repeated addition of 0.025 ml of 2% NaOH in water, 8 ml of each drink until pH 7. The crown of each of the four human molars was sectioned from the root and subjected to a cut in the mesio-distal direction. It was designed a rectangular window display of enamel in the buccal and lingual faces. Using the knife Isomet 1000 we obtained two rectangular display windows, which were cut in half to obtain sixteen specimens. The area outside the display window was covered with varnish. The specimens painted with the same color of varnish were collected and formed four groups. It was assured the inclusion of a specimen from each molar in each group. Randomly, three groups were subjected to four cycles of demineralization / remineralization and the fourth group continued to be immersed in artificial saliva. At the end of the experimental procedure, four specimens from each group were removed and prepared to the SEM surface analysis. Results: The drink Coca-Cola has the lowest initial pH (pH = 2.54) and drink Red Bull has a higher buffer capacity. The selected acidic drinks promoted wear at the surface of human enamel and artificial saliva did not cause changes in the enamel. Conclusion: The erosive potential of the selected acidic drinks was confirmed in our study.
Dental erosion is a chronic process, painless with irreversible loss of tooth tissue due to a chemical process that does not involve microorganisms. Objective: This in vitro study intends to evaluate the effects of erosion made by Coca Cola, Só Laranja and Red Bull, on the surface of samples of human enamel through the observation on the scanning electron microscope (SEM). Materials and methods: The pH of each beverage was determined using a potentiometer pH. The buffer capacity was determined after repeated addition of 0.025 ml of 2% NaOH in water, 8 ml of each drink until pH 7. The crown of each of the four human molars was sectioned from the root and subjected to a cut in the mesio-distal direction. It was designed a rectangular window display of enamel in the buccal and lingual faces. Using the knife Isomet 1000 we obtained two rectangular display windows, which were cut in half to obtain sixteen specimens. The area outside the display window was covered with varnish. The specimens painted with the same color of varnish were collected and formed four groups. It was assured the inclusion of a specimen from each molar in each group. Randomly, three groups were subjected to four cycles of demineralization / remineralization and the fourth group continued to be immersed in artificial saliva. At the end of the experimental procedure, four specimens from each group were removed and prepared to the SEM surface analysis. Results: The drink Coca-Cola has the lowest initial pH (pH = 2.54) and drink Red Bull has a higher buffer capacity. The selected acidic drinks promoted wear at the surface of human enamel and artificial saliva did not cause changes in the enamel. Conclusion: The erosive potential of the selected acidic drinks was confirmed in our study.
Descrição
Tese de mestrado, Medicina Dentária, Universidade de Lisboa, Faculdade de Medicina Dentária, 2011
Palavras-chave
Cariologia Teses de mestrado - 2011