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Analyzing the safety of the parasiticide fungus Mucor circinelloides: first insights on its virulence profile and interactions with the avian gut microbial community

2024-05-02Journal article Open access
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dc.contributor.authorLozano, João
dc.contributor.authorCunha, Eva
dc.contributor.authorAlmeida, Cristina
dc.contributor.authorNunes, Mónica
dc.contributor.authorDias, Ricardo
dc.contributor.authorVicente, Eduardo
dc.contributor.authorSebastião, Daniela
dc.contributor.authorHenriques, Sérgio
dc.contributor.authorMadeira de Carvalho, Luís
dc.contributor.authorPaz-Silva, Adolfo
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2024-06-29T14:14:35Z
dc.date.available2024-06-29T14:14:35Z
dc.date.issued2024-05-02
dc.descriptionResearch Areas: Microbiologypt_PT
dc.description.abstractParasiticide fungi are considered an accurate, sustainable, and safe solution for the biocontrol of animal gastrointestinal (GI) parasites. This research provides an initial characterization of the virulence of the native parasiticide fungus Mucor circinelloides (FMV-FR1) and an assessment of its impact on birds’ gut microbes. The genome of this fungus was sequenced to identify the genes coding for virulence factors. Also, this fungus was checked for the phenotypic expression of proteinase, lecithinase, DNase, gelatinase, hemolysin, and biofilm production. Finally, an in vivo trial was developed based on feeding M. circinelloides spores to laying hens and peacocks three times a week. Bird feces were collected for 3 months, with total genomic DNA being extracted and subjected to long-read 16S and 25S-28S sequencing. Genes coding for an iron permease (FTR1), iron receptors (FOB1 and FOB2), ADP-ribosylation factors (ARFs) (ARF2 and ARF6), and a GTPase (CDC42) were identified in this M. circinelloides genome. Also, this fungus was positive only for lecithinase activity. The field trial revealed a fecal microbiome dominated by Firmicutes and Proteobacteria in laying hens, and Firmicutes and Bacteroidetes in peacocks, whereas the fecal mycobiome of both bird species was mainly composed of Ascomycetes and Basidiomycetes fungi. Bacterial and fungal alpha-diversities did not differ between sampling time points after M. circinelloides administrations (P = 0.62 and P = 0.15, respectively). Although findings from this research suggest the lack of virulence of this M. circinelloides parasiticide isolate, more complementary in vitro and in vivo research is needed to conclude about the safety of its administration to birds, aiming at controlling their GI parasites.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLozano J, Cunha E, Almeida C, Nunes M, Dias R, Vicente E, Sebastiao D, Henriques S, de Carvalho LM, Paz-Silva A, Oliveira M. 2024. Analyzing the safety of the parasiticide fungus Mucor circinelloides: first insights on its virulence profile and interactions with the avian gut microbial community. Microbiology Spectrum, 12(5). doi:10.1128/spectrum.04078-23
dc.identifier.doi10.1128/spectrum.04078-23pt_PT
dc.identifier.issn2165-0497
dc.identifier.urihttp://hdl.handle.net/10400.5/31193
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationPID2020-120208RB-I00 (MCINN, Spain; FEDER)pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relationNovo: Integrated control of avian gastrointestinal parasites: optimizing their coprological diagnosis and biocontrol using predatory fungi. Inicial: Gastrointestinal Parasites of Domestic Birds and their Biological Control with Predatory Fungi
dc.relation.publisherversionhttps://journals.asm.org/doi/pdf/10.1128/spectrum.04078-23pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAvian parasitologypt_PT
dc.subjectParasiticide fungipt_PT
dc.subjectVirulence factorspt_PT
dc.subjectMicrobiomept_PT
dc.subjectMycobiomept_PT
dc.titleAnalyzing the safety of the parasiticide fungus Mucor circinelloides: first insights on its virulence profile and interactions with the avian gut microbial communitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardTitleNovo: Integrated control of avian gastrointestinal parasites: optimizing their coprological diagnosis and biocontrol using predatory fungi. Inicial: Gastrointestinal Parasites of Domestic Birds and their Biological Control with Predatory Fungi
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0059%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/2020.09037.BD/PT
oaire.citation.conferencePlaceWASHINGTON, DCpt_PT
oaire.citation.issue5pt_PT
oaire.citation.titleMicrobiology Spectrumpt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNamePestana Lozano
person.familyNameGonçalves da Cunha
person.familyNameMadeira de Carvalho
person.familyNamePaz-Silva
person.familyNameOliveira
person.givenNameJoão Miguel
person.givenNameEva Sofia
person.givenNameLuís Manuel
person.givenNameAdolfo
person.givenNameManuela
person.identifier.ciencia-idA81D-BFE3-3D35
person.identifier.ciencia-id9811-7B6B-E2B9
person.identifier.ciencia-id9416-643A-4E0C
person.identifier.ciencia-idBE1E-2B3B-92D2
person.identifier.orcid0000-0001-5390-6998
person.identifier.orcid0000-0002-5641-4400
person.identifier.orcid0000-0002-7548-667X
person.identifier.orcid0000-0002-0208-3861
person.identifier.orcid0000-0002-3910-1525
person.identifier.ridI-2788-2015
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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