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Engineering tissue with acoustic manipulation
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Resumo(s)
Tissue engineering is an emerging technology that addresses the limitations of organ transplantation by providing strategies to regenerate, repair or replace damaged tissues. A key challenge in this field is the ability to control cell organization, which is essential for the functionality of anisotropic tissues, such as cardiac tissue. Acoustic manipulation has emerged as a non-invasive, label-free and high-resolution technique to align cells and spheroids. This dissertation explores the alignment of Human Dermal Fibroblasts (HDF) spheroids under acoustic fields, with the aim of developing an optimized setup for this purpose. The Six-Sigma Methodology was implemented to identify customer requirements, translate them into measurable parameters, analyse them and design possible concepts. The different factors that influence the acoustic field were studied through experiments with both microparticles and spheroids, whose movement was assessed using different software tools, such as ImageJ, Python, MATLAB and Microsoft Excel. In addition, amplitude measurements were conducted as a method to analyse losses of energy of the ultrasound waves after propagation. The knowledge acquired from the experiments performed, regarding the influence of different parameters on the alignment of HDF spheroids, allowed the development of a House of Quality. Furthermore, design concepts for an optimized setup were developed in SolidWorks and pilot runs were conducted with the most promising alternatives. Additionally, a Design of Experiments was performed to evaluate the performance of the selected design. This work advances the understanding of acoustic manipulation in tissue engineering, providing experimental insights, analytical tools and design strategies that support the development of more effective alignment systems.
Descrição
Tese de Mestrado, Engenharia Biomédica e Biofísica, 2026, Universidade de Lisboa, Faculdade de Ciências
Palavras-chave
Acoustic Manipulation Tissue Engineering Alignment Amplitude Wave Propagation