Authors
Abstract(s)
Os gambás (Didelphis spp.) são mamíferos marsupiais que ocorrem ao longo do conti-nente americano e que, devido à cada vez maior destruição dos seus habitats e aproximação dos centros urbanos, são hoje considerados animais sinantrópicos.
Estes animais constituem um importante reservatório natural de Trypanosoma cruzi, pro-tozoário agente da Doença de Chagas, considerada, pela Organização Mundial de Saúde, uma Doença Tropical Negligenciada.
Os estudos sobre a fauna helmintológica dos gambás restringem-se sobretudo ao gambá-da-virgínia (Didelphis virginiana) e ao gambá-comum (Didelphis marsupialis). Os registos feitos de helmintes em gambás no Brasil são esporádicos, dispersos e também muito variáveis, con-forme as dinâmicas ecológicas nas diferentes regiões e biomas. No Estado de São Paulo, ocor-rem as espécies gambá-de-orelha-branca (Didelphis albiventris) e gambá-de-orelha-preta (Didel-phis aurita).
Os objetivos deste trabalho foram averiguar a existência de T. cruzi, bem como pesquisar helmintes nos gambás que deram entrada na Associação Mata Ciliar (Jundiaí, São Paulo, Brasil), oriundos do município de Jundiaí e municípios circundantes. Para tal, foram colhidas amostras nos gambás-de-orelha-branca e de orelha-preta em recuperação (n=20) e realizadas necrópsias (n=11), na Associação Mata Ciliar, entre os dias 10 e 19 de Maio de 2019, para posterior proces-samento das amostras na Faculdade de Ciências Agrárias e Veterinárias - UNESP, Jaboticabal, São Paulo. As análises feitas por RCP, bem como os esfregaços de conteúdo perianal e sangue, revelaram-se negativos para a pesquisa de T. cruzi. A pesquisa de helmintes revelou que 5 dos 10 animais sujeitos a necrópsia estavam infetados. Os helmintes encontrados e respetivas pre-valências foram Cruzia sp. (40%, 4/10), Strongyloides sp. (40%, 4/10), Trichuris sp. (30%, 3/10), Turgida sp. (10%,1/10), Oligacanthorhynchus sp. (10%, 1/10) e Cestoda (40%, 4/10). Este estudo representa, assim, um importante contributo para o conhecimento da fauna helmintológica dos gambás do município de Jundiaí e municípios circundantes e poderá constituir uma base para investigações futuras sobre este tema.
ABSTRACT - Research of Trypanosoma cruzi and report of occurrence of gastrointestinal hel-minths in White-eared Opossum (Didelphis albiventris) and Brazilian Common Opossum (Didelphis aurita) in a wildlife rehabilitation center in the municipality of Jundiaí, State of São Paulo, Brazil - Opossums (Didelphis spp.) are marsupial mammals that occur throughout the American continent. Nowadays they are considered synanthropic animals due to the increasing destruction of their habitats and the proximity of urban centers. These animals are an important reservoir of Trypanosoma cruzi, a protozoan agent of Chagas disease which is considered a Neglected Tropical Disease by the World Health Organi-zation. Studies on the helminthological fauna of opossums are mainly restricted to the Virginia opossum (Didelphis virginiana) and the common opossum (Didelphis marsupialis). The records of helminths from opossums in Brazil are sporadic, dispersed and very variable, according to the ecological dynamics in the different regions and biomes. In the state of São Paulo, the species that occur are the White-eared Opossum (Didelphis albiventris) and Brazilian Common Opossum (Didelphis aurita). The objectives of this work were to investigate the existence of T. cruzi, as well as to search for helminths in the opossums admitted to the Associação Mata Ciliar (Jundiaí, São Paulo, Brazil), from the municipality of Jundiaí and surrounding municipalities. To this end, samples were collected from opossums in rehabilitation (n=20) and necropsies (N=11) were performed at As-sociação Mata Ciliar between 10th and 19th May 2019, for further sample processing at Faculdade de Ciências Agrárias e Veterinárias - UNESP, Jaboticabal, São Paulo. PCR analyzes, as well as smears of perianal content and blood for T. cruzi research, were negative. The helminth research revealed that 5 out of 10 necropsied opossums were infected. The helminths found and their prevalence were Cruzia sp. (40%, 4/10), Strongyloides sp. (40%, 4/10), Trichuris sp. (30%, 3/10), Turgida sp. (10%, 1/10), Oligacanthorhynchus sp. (10%, 1/10) and Cestoda (40%, 4/10). This study thus constitutes an important contribution to the knowledge of the helmintho-logical fauna of opossums in the municipality of Jundiaí and surrounding municipalities and could provide a basis for future research on this topic.
ABSTRACT - Research of Trypanosoma cruzi and report of occurrence of gastrointestinal hel-minths in White-eared Opossum (Didelphis albiventris) and Brazilian Common Opossum (Didelphis aurita) in a wildlife rehabilitation center in the municipality of Jundiaí, State of São Paulo, Brazil - Opossums (Didelphis spp.) are marsupial mammals that occur throughout the American continent. Nowadays they are considered synanthropic animals due to the increasing destruction of their habitats and the proximity of urban centers. These animals are an important reservoir of Trypanosoma cruzi, a protozoan agent of Chagas disease which is considered a Neglected Tropical Disease by the World Health Organi-zation. Studies on the helminthological fauna of opossums are mainly restricted to the Virginia opossum (Didelphis virginiana) and the common opossum (Didelphis marsupialis). The records of helminths from opossums in Brazil are sporadic, dispersed and very variable, according to the ecological dynamics in the different regions and biomes. In the state of São Paulo, the species that occur are the White-eared Opossum (Didelphis albiventris) and Brazilian Common Opossum (Didelphis aurita). The objectives of this work were to investigate the existence of T. cruzi, as well as to search for helminths in the opossums admitted to the Associação Mata Ciliar (Jundiaí, São Paulo, Brazil), from the municipality of Jundiaí and surrounding municipalities. To this end, samples were collected from opossums in rehabilitation (n=20) and necropsies (N=11) were performed at As-sociação Mata Ciliar between 10th and 19th May 2019, for further sample processing at Faculdade de Ciências Agrárias e Veterinárias - UNESP, Jaboticabal, São Paulo. PCR analyzes, as well as smears of perianal content and blood for T. cruzi research, were negative. The helminth research revealed that 5 out of 10 necropsied opossums were infected. The helminths found and their prevalence were Cruzia sp. (40%, 4/10), Strongyloides sp. (40%, 4/10), Trichuris sp. (30%, 3/10), Turgida sp. (10%, 1/10), Oligacanthorhynchus sp. (10%, 1/10) and Cestoda (40%, 4/10). This study thus constitutes an important contribution to the knowledge of the helmintho-logical fauna of opossums in the municipality of Jundiaí and surrounding municipalities and could provide a basis for future research on this topic.
Description
Dissertação de Mestrado Integrado em Medicina Veterinária
Keywords
Gambás Didelphis Brasil Trypanosoma cruzi Helmintes Opossums Didelphis Brazil Trypanosoma cruzi Helminths
Pedagogical Context
Citation
Cruz LP 2021. Pesquisa de Trypanosoma cruzi e relato de ocorrência de helmintes gastrointestinais em gambá-de-orelha-branca (Didelphis albiventris) e gambá-de-orelha-preta (Didelphis aurita) num CRAS do município de Jundiaí, estado de São Paulo, Brasil [dissertação de mestrado]. Lisboa: FMV-Universidade de Lisboa.
Publisher
Universidade de Lisboa, Faculdade de Medicina Veterinária