A carregar...
Publicação
Effects of acetylcholine on an animal mode of inflammation
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 134.13 KB | Adobe PDF |
Orientador(es)
Resumo(s)
Acetylcholinesterase (AChE) is found both on the membranes of neuronal and non-neuronal cells. In this study we
performed intravenous administrations of velnacrine (VLN) and acetylcholine (ACh), respectively,AChE inhibitor and substrate, in an animal model of lipopolysaccharide (LPS)-induced inflammation in Wistar rats. Using intravital microscopy the number of rolling and adherent leukocytes in post-capillary venules was monitorized and blood samples were collected for TNF-α plasma concentrations determination. Our results showed that in presence of LPS, ACh has an anti-inflammatory effect, seen by a decrease in TNF-α plasma levels and maintains the number of rolling and adherent leukocytes. The presence of VLN before LPS almost blocked the LPS-induced rolling and TNF-α releasing. Thereby VLN seems to have, like ACh, an anti-inflammatory effect by diminishing TNF-α concentrations.
Descrição
© 2013 – IOS Press and the authors. All rights reserved
Palavras-chave
Inflammation Acetylcholine TNF-α Velnacrine Rolling leukocytes Intravital microscopy
Contexto Educativo
Citação
Clinical Hemorheology and Microcirculation 53 (2013) 209–216