Publication
Endothelin-1 effects on spontaneous oscillations in choroidal arterioles
| dc.contributor.author | Delgado, Esmeralda | |
| dc.contributor.author | Marques-Neves, Carlos | |
| dc.contributor.author | Rocha, Isabel | |
| dc.contributor.author | Sales-Luís, José | |
| dc.contributor.author | Silva-Carvalho, Luis | |
| dc.date.accessioned | 2012-07-23T10:42:38Z | |
| dc.date.available | 2012-07-23T10:42:38Z | |
| dc.date.issued | 2010 | |
| dc.description | © 2009 The Authors Journal compilation © 2009 Acta Ophthalmol | por |
| dc.description.abstract | Purpose: This study characterizes the effects of endothelin-1 (ET-1) on the perfusion pressure of the choroidal vasculature using in situ perfused isolated rabbit eyes. Methods: Rabbit external ophthalmic arteries (n = 12) in a head-mounted preparation were cannulated and perfused with warmed tyrode. The three-way polypropylene catheter was further connected to a pressure transducer and the effect of intraluminal pressure as a measure of total vascular resistance was assessed. Response curves to intra-arterial injections of ET-1 (group A; n = 6) and to an intravitreal injection followed by an intra-arterial injection of ET-1 (group B; n = 6) were obtained. Data were studied using paired t-test and fast Fourier transform. Results: Before any drugs were administered, spontaneous oscillations were observed in the 12 rabbit models. In group A, ET-1 induced a short and weak vasodilating effect followed by a strong and long-lasting vasoconstrictor tone. Vasomotion became more evident, showing a higher frequency and shorter amplitude of oscillations. In group, B the intravitreal injection produced no significant changes in registered pressure or vasomotion characteristics. The intra-arterial injection produced effects similar to those observed in group A. Conclusion: Our study has three main findings: (i) the choroidal vasculature demonstrated spontaneous oscillations in perfusion pressure in basal conditions in all rabbit eye models; (ii) ET-1 applied intra-arterially induced a short drop in perfusion pressure followed by a long withstanding contraction; and (iii) intra-arterial ET-1 modulated the frequency and amplitude of the spontaneous oscillations, causing a faster rate of pulsatility. | eng |
| dc.description.sponsorship | This work was supported by Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA) | eng |
| dc.identifier.citation | Acta Ophthalmol. 2010: 88: 742–747 | por |
| dc.identifier.issn | 1755-375X | |
| dc.identifier.uri | http://dx.doi.org/10.1111/j.1755-3768.2009.01537.x | |
| dc.identifier.uri | http://hdl.handle.net/10451/6734 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Wiley-Blackwell | por |
| dc.relation.publisherversion | The definitive version is available at www3.interscience.wiley.com | eng |
| dc.subject | Choroidal vasomotion | eng |
| dc.subject | Endothelin-1 | eng |
| dc.subject | Isolated perfused rabbit eye | eng |
| dc.subject | Spontaneous oscillations | eng |
| dc.title | Endothelin-1 effects on spontaneous oscillations in choroidal arterioles | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 747 | por |
| oaire.citation.startPage | 742 | por |
| oaire.citation.title | Acta Ophthalmologica | eng |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |