Endothelin-1 effects on spontaneous oscillations in choroidal arterioles

Delgado, Esmeralda; Marques-Neves, Carlos; Rocha, Isabel; Sales-Luís, José; Silva-Carvalho, Luis

http://hdl.handle.net/10451/6734

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Authors

Delgado, Esmeralda

Marques-Neves, Carlos

Rocha, Isabel

Sales-Luís, José

Silva-Carvalho, Luis

Abstract(s)

Purpose: This study characterizes the effects of endothelin-1 (ET-1) on the perfusion pressure of the choroidal vasculature using in situ perfused isolated rabbit eyes. Methods: Rabbit external ophthalmic arteries (n = 12) in a head-mounted preparation were cannulated and perfused with warmed tyrode. The three-way polypropylene catheter was further connected to a pressure transducer and the effect of intraluminal pressure as a measure of total vascular resistance was assessed. Response curves to intra-arterial injections of ET-1 (group A; n = 6) and to an intravitreal injection followed by an intra-arterial injection of ET-1 (group B; n = 6) were obtained. Data were studied using paired t-test and fast Fourier transform. Results: Before any drugs were administered, spontaneous oscillations were observed in the 12 rabbit models. In group A, ET-1 induced a short and weak vasodilating effect followed by a strong and long-lasting vasoconstrictor tone. Vasomotion became more evident, showing a higher frequency and shorter amplitude of oscillations. In group, B the intravitreal injection produced no significant changes in registered pressure or vasomotion characteristics. The intra-arterial injection produced effects similar to those observed in group A. Conclusion: Our study has three main findings: (i) the choroidal vasculature demonstrated spontaneous oscillations in perfusion pressure in basal conditions in all rabbit eye models; (ii) ET-1 applied intra-arterially induced a short drop in perfusion pressure followed by a long withstanding contraction; and (iii) intra-arterial ET-1 modulated the frequency and amplitude of the spontaneous oscillations, causing a faster rate of pulsatility.