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Publicação
Contributos para o melhoramento em sobreiro: instalação de rebentos in vitro visando a obtenção de híbridos. Fenologia da espécie e ontogenia da sua reprodução sexuada
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Resumo(s)
Focusing cork-oak breeding, we assayed in vitro establishments of apical and nodal segments from mature trees of Quercus suber L. collected directly from the field, in order to reach a prompt method for controlled pollinations of female flowers established in vitro. The plant material used for in vitro establishment was sampled between April and September 2013 from a plot in south of Setúbal peninsula. In this context, phenology was monitored in a population of 31 trees from the same plot and the ontogenic development of reproductive female structures and acorns of one tree was histologically studied. The in vitro development of explants was very difficult, due to contaminations, tissue oxidation and low reactivity of explants, looking more promising the use of shoots in mouse-ear phenological stage (at the beginning of axis elongation) collected in spring, superficial sterilizations with solutions of calcium hypochlorite and fungicides and culture in agar water, with foliar fertilizer applications, prior being transferred to nutritive medium. Phenological data showed intraspecific variation and sporadic flowering, outside spring. Relationships between phenology and ontogeny were recognized, and a long progamic phase (80 days) and an early full differentiation of embryos have been documented
Descrição
Mestrado em Engenharia Agronómica - Horticultura e Viticultura - Instituto Superior de Agronomia
Palavras-chave
Quercus suber in vitro establishment reproductive biology phenology ontogeny histology
Contexto Educativo
Citação
Luz, F.M.C.P.R. - Contributos para o melhoramento em sobreiro: instalação de rebentos in vitro visando a obtenção de híbridos. Fenologia da espécie e ontogenia da sua reprodução sexuada. Lisboa: ISA, 2015, 100 p.