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Comparação do efeito bactericida da activação manual e da activação ultrassónica do NaOCI a 5,25% contra Enterococcus faecalis
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Introdução: Neste estudo propomos-nos a estudar a irrigação, por ser a fase mais importante na remoção de microrganismos do sistema canalar, permitindo uma limpeza e desinfecção intracanalar compatível com sucesso endodôntico. Comparámos entre si, técnicas de activação manual e de activação mecânica, ambas com o NaOCl a 5,25% na sua capacidade bactericida contra Enterococcus faecalis (E. faecalis) ATCC 51299. Materiais e Métodos: Uma amostra de 66 dentes unirradiculares foi preparada com instrumentos rotativos da ProTaper Universal®. A smear layer foi removida com ácido cítrico a 10% e NaOCl a 5,25%. Esterilizaram-se os dentes em autoclave com um ciclo de 15 minutos a 121º C. Cada grupo experimental foi constituído por 20 dentes e dois grupos de controlo (positivo e negativo) cada um constituído por 3 dentes. Inocularam-se os dentes dos grupos experimentais e os do controlo positivo com com uma suspensão bacterina de E. faecalis. De seguida trataram-se os dentes de cada grupo da seguinte forma. Grupo1: Irrigação com NaOCl a 5,25% activação manual com seringa. Grupo 2: Irrigação com NaOCl a 5,25% com agitação manual-dinâmica. Grupo 3: Irrigação com NaOCl a 5,25% com ProUltra® PiezoFlow™ Ultrasonic Irrigation. A desinfecção foi avaliada pela presença ou ausência de turvação, após colheita da amostra com cones de papel e introduzidos em meio BHI estéril, durante 72 horas. Resultados: Podemos verificar que a agitação manual dinâmica tem valores de eficácia superiores a ambas as técnicas, e que a irrigação ultrassónica passiva tem eficácia superior à activação manual com seringa e inferior eficácia que a agitação manual dinâmica. Conclusão: Neste estudo não conseguimos verificar se as nossas hipóteses foram rejeitadas ou não, devido à contaminação proveniente de uma fonte externa desconhecida, revelando-se ser necessária a realização de um novo ensaio laboratorial para se obterem resultados conclusivos.
Introduction: In this study, we propose to examine the irrigation process, the most important phase in the removal of microorganisms from the channel system, allowing the cleaning and disinfection of the intracanal compatible with endodontic success. We compared with each other, manual activation techniques and mechanical activation, both with 5.25% NaOCl in their bactericidal ability against ATCC 51299 Enterococcus faecalis (E. faecalis). Materials and Methods: A sample of 66 single-rooted teeth were prepared with rotary instruments with ProTaper Universal®. The smear layer was removed with 10% (m/v) citric acid and NaOCl 5.25%. Teeth were sterilized in an autoclave with a cycle of 15 minutes at 121ºC. Each experimental group consisted of 20 teeth and two control groups (positive and negative) consisted of 3 teeth. Both the experimental groups and the positive control group teeth were inoculated with Enterococcus faecalis. Then, each teeth’s group was treated following the next scheme. Group 1: Irrigation with NaOCl 5.25% with manual activation with a syringe. Group 2: Irrigation with NaOCl 5.25% with manual-dynamic irrigation. Group 3: Irrigation with NaOCl 5.25% (m/v) with ProUltra PiezoFlow ® ™ Ultrasonic Irrigation. The disinfection was evaluated by sampling with paper cones that were incubated in sterile BHI media for 72 hours. Results: We found that the manual-dynamic irrigation has superior efficacy values for both techniques, and the passive ultrasonic irrigation has superior efficacy to the manual activation using a syringe and less effective as manual-dynamic irrigation. Conclusion: In this study we could not verify that our hypotheses were right, due to a contamination from an unknown outside source, being necessary to perform new laboratory tests in order to achieve our objectives.
Introduction: In this study, we propose to examine the irrigation process, the most important phase in the removal of microorganisms from the channel system, allowing the cleaning and disinfection of the intracanal compatible with endodontic success. We compared with each other, manual activation techniques and mechanical activation, both with 5.25% NaOCl in their bactericidal ability against ATCC 51299 Enterococcus faecalis (E. faecalis). Materials and Methods: A sample of 66 single-rooted teeth were prepared with rotary instruments with ProTaper Universal®. The smear layer was removed with 10% (m/v) citric acid and NaOCl 5.25%. Teeth were sterilized in an autoclave with a cycle of 15 minutes at 121ºC. Each experimental group consisted of 20 teeth and two control groups (positive and negative) consisted of 3 teeth. Both the experimental groups and the positive control group teeth were inoculated with Enterococcus faecalis. Then, each teeth’s group was treated following the next scheme. Group 1: Irrigation with NaOCl 5.25% with manual activation with a syringe. Group 2: Irrigation with NaOCl 5.25% with manual-dynamic irrigation. Group 3: Irrigation with NaOCl 5.25% (m/v) with ProUltra PiezoFlow ® ™ Ultrasonic Irrigation. The disinfection was evaluated by sampling with paper cones that were incubated in sterile BHI media for 72 hours. Results: We found that the manual-dynamic irrigation has superior efficacy values for both techniques, and the passive ultrasonic irrigation has superior efficacy to the manual activation using a syringe and less effective as manual-dynamic irrigation. Conclusion: In this study we could not verify that our hypotheses were right, due to a contamination from an unknown outside source, being necessary to perform new laboratory tests in order to achieve our objectives.
Descrição
Tese de mestrado, Medicina Dentária, Universidade de Lisboa, Faculdade de Medicina Dentária, 2011
Palavras-chave
Endodontia Teses de mestrado - 2011