Loading...
Publication
How crystal clear is it? : estudo morfométrico e histopatológico do coração de gatos com cardiomiopatia hipertrófica
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 2.68 MB | Adobe PDF |
Authors
Abstract(s)
A cardiomiopatia hipertrófica (HCM) é a cardiopatia mais frequente em gatos. O seu diagnóstico é predominantemente clínico, em vida, baseado no exame ecocardiográfico, seguindo-se o seu estadiamento de acordo com os restantes sinais clínicos e sintomas do animal. Em contrapartida, os critérios de diagnóstico pós-morte desta afeção não estão solidamente estabelecidos, sendo que muitos patologistas se baseiam no peso do coração e na avaliação macroscópica subjetiva e pessoal de alterações histopatológicas de tecido cardíaco. Foram recolhidos corações de gatos necropsiados no Serviço de Anatomia Patológica da FMV-ULisboa (n=32). Estes foram divididos em grupo controlo e grupo doente/HCM, de acordo com o peso do coração. Seguidamente, procedeu-se à análise morfométrica macroscópica e à avaliação histopatológica de amostras de miocárdio. Foi realizada coloração imunohistoquímica para troponina I cardíaca (cTnI) e, finalmente, efetuou-se a medição da espessura dos cardiomiócitos. Observaram-se diferenças estatisticamente significativas entre os grupos controlo e HCM na maior parte das variáveis morfométricas analisadas. No que diz respeito aos achados histopatológicos, a única diferença estatisticamente significativa foi no grau de fibrose da parede do ventrículo esquerdo. Além disso, a marcação imunohistoquímica para cTnI não detetou diferenças significativas entre tecido saudável e tecido lesionado. A espessura dos cardiomiócitos revelou diferenças significativas entre os dois grupos. Em conclusão, este estudo resultou num conjunto de observações que permitem uma melhor e mais completa compreensão morfométrica e histopatológica da HCM em gatos. Mais estudos permitirão uma melhor e mais completa compreensão da patologia clínica e histopatologia da HCM felina
ABSTRACT - HOW CRYSTAL CLEAR IS IT? – MORPHOMETRIC AND HISTOPATHOLOGICAL STUDY OF THE HEART OF CATS WITH HYPERTROPHIC CARDIOMYOPATHY - Hypertrophic cardiomyopathy (HCM) is the most common heart disease in cats. Its diagnosis is predominantly clinical, in vivo, based on echocardiographic examination, followed by staging according to the animal's clinical signs and symptoms. In contrast, the postmortem diagnostic criteria of this condition are not solidly established, and, therefore, many pathologists rely on the weight of the heart and on the subjective assessment of histopathological changes of cardiac tissues samples. Hearts were collected from cats presented for necropsy at the Pathological Anatomy Service of FMV-ULisboa (n=32) and divided into a control group and a sick/HCM group according to heart weight. Macroscopic morphometric evaluation of these hearts was then carried out, as well as histopathological analysis of myocardial samples. Immunohistocemical staining for cardiac troponin I (cTnI) was performed and, finally, the thickness of the cardiomyocytes was measured. Statistically significant differences were observed between the control and HCM groups in most of the morphometric variables analyzed. With regard to histopathological findings, the only statistically significant difference was in the degree of fibrosis of the left ventricular wall, in one of the scenarios. Furthermore, immunohistochemical staining for cTnI did not effectively detect differences between healthy and injured cardiac tissue. Regarding the determination of cardiomyocyte thickness, significant difference values were obtained between the two groups. In conclusion, this study resulted in a set of observations that allow a better and more complete morphometric and histopathological understanding of HCM in cats. Further studies will allow for a better and more complete understanding of the clinical pathology and histopathology of feline HCM
ABSTRACT - HOW CRYSTAL CLEAR IS IT? – MORPHOMETRIC AND HISTOPATHOLOGICAL STUDY OF THE HEART OF CATS WITH HYPERTROPHIC CARDIOMYOPATHY - Hypertrophic cardiomyopathy (HCM) is the most common heart disease in cats. Its diagnosis is predominantly clinical, in vivo, based on echocardiographic examination, followed by staging according to the animal's clinical signs and symptoms. In contrast, the postmortem diagnostic criteria of this condition are not solidly established, and, therefore, many pathologists rely on the weight of the heart and on the subjective assessment of histopathological changes of cardiac tissues samples. Hearts were collected from cats presented for necropsy at the Pathological Anatomy Service of FMV-ULisboa (n=32) and divided into a control group and a sick/HCM group according to heart weight. Macroscopic morphometric evaluation of these hearts was then carried out, as well as histopathological analysis of myocardial samples. Immunohistocemical staining for cardiac troponin I (cTnI) was performed and, finally, the thickness of the cardiomyocytes was measured. Statistically significant differences were observed between the control and HCM groups in most of the morphometric variables analyzed. With regard to histopathological findings, the only statistically significant difference was in the degree of fibrosis of the left ventricular wall, in one of the scenarios. Furthermore, immunohistochemical staining for cTnI did not effectively detect differences between healthy and injured cardiac tissue. Regarding the determination of cardiomyocyte thickness, significant difference values were obtained between the two groups. In conclusion, this study resulted in a set of observations that allow a better and more complete morphometric and histopathological understanding of HCM in cats. Further studies will allow for a better and more complete understanding of the clinical pathology and histopathology of feline HCM
Description
Dissertação de Mestrado Integrado em Medicina Veterinária, área científica de Sanidade Animal
Keywords
Cardiomiopatia hipertrófica Gato Miocárdio Morfometria Histopatologia Cardiomiócitos Hypertrophic cardiomyopathy Cat Myocardium Morphometry Histopathology Cardiomyocytes
Pedagogical Context
Citation
Lopes JFC. 2024. How crystal clear is it? : estudo morfométrico e histopatológico do coração de gatos com cardiomiopatia hipertrófica [dissertação de mestrado]. Lisboa: FMV-Universidade de Lisboa
Publisher
Universidade de Lisboa, Faculdade de Medicina Veterinária