Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells

Cal, Pedro M.S.D.; Sieglitz, Florian; Santos, Fábio; Parente Carvalho, Cátia; Guerreiro, Ana; Bertoldo, Jean B.; Pischel, Uwe; Gois, Pedro M. P.; Bernardes, Gonçalo J. L.

http://hdl.handle.net/10451/51441

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Autores

Cal, Pedro M.S.D.

Sieglitz, Florian

Santos, Fábio

Parente Carvalho, Cátia

Bernardes, Gonçalo J. L.

Resumo(s)

Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells.