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Site-selective installation of BASHY fluorescent dyes to Annexin V for targeted detection of apoptotic cells
Publication . Cal, Pedro M.S.D.; Sieglitz, Florian; Santos, Fábio; Parente Carvalho, Cátia; Guerreiro, Ana; Bertoldo, Jean B.; Pischel, Uwe; Gois, Pedro M. P.; Bernardes, Gonçalo J. L.
Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes and their use for site-selective labelling of Annexin V. The Annexin V-BASHY conjugate maintained function and fluorescence as demonstrated by the targeted detection of apoptotic cells.

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Funding agency

Fundação para a Ciência e a Tecnologia

Funding programme

3599-PPCDT

Funding Award Number

PTDC/QUI-QUI/118315/2010

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