Publicação
Investigating the interaction between CFP-STAT3-CTAD and YFP-P300-CH1
| datacite.subject.fos | Ciências da Saúde | pt_PT |
| dc.contributor.advisor | Wilderspin, Andy | |
| dc.contributor.advisor | Borralho, Pedro | |
| dc.contributor.author | Gaspar, Catarina João Silva | |
| dc.date.accessioned | 2017-11-07T12:59:21Z | |
| dc.date.available | 2017-11-07T12:59:21Z | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2013 | |
| dc.description | Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014 | pt_PT |
| dc.description.abstract | Previous studies have shown evidence that STAT3CTAD and p300CH1 may interact in cancer related pathways. In this study, a GFP variant was fused to the N-termini of each protein. CFP was fused to the STAT3CTAD and YFP was fused to p300CH1. In this way, a possible interaction between the two proteins could be detected, using FRET as a screening method. The preliminary titration results demonstrate a FRET signal between the two proteins. However, additional studies would have to be performed in order to attribute the observed FRET signal to a protein-protein interaction. When validated, this assay could be used to test compounds as inhibitors of the proposed interaction. | pt_PT |
| dc.description.sponsorship | Department of Pharmaceutical and Biological Chemistry, UCL School of Pharmacy, London. | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10451/29619 | |
| dc.language.iso | eng | pt_PT |
| dc.subject | Mestrado Integrado - 2014 | pt_PT |
| dc.title | Investigating the interaction between CFP-STAT3-CTAD and YFP-P300-CH1 | pt_PT |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | restrictedAccess | pt_PT |
| rcaap.type | masterThesis | pt_PT |
| thesis.degree.name | Mestrado Integrado em Ciências Farmacêuticas | pt_PT |