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Investigating the interaction between CFP-STAT3-CTAD and YFP-P300-CH1
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Resumo(s)
Previous studies have shown evidence that STAT3CTAD and p300CH1 may interact in cancer related pathways. In this study, a GFP variant was fused to the N-termini of each protein. CFP was fused to the STAT3CTAD and YFP was fused to p300CH1. In this way, a possible interaction between the two proteins could be detected, using FRET as a screening method.
The preliminary titration results demonstrate a FRET signal between the two proteins. However, additional studies would have to be performed in order to attribute the observed FRET signal to a protein-protein interaction. When validated, this assay could be used to test compounds as inhibitors of the proposed interaction.
Descrição
Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014
Palavras-chave
Mestrado Integrado - 2014