Publicação
Functional characterization of cork oak AINTEGUMENTA in suberization and periderm development via genetic complementation approaches
| dc.contributor.advisor | Miguel,Célia Maria Romba Rodrigues | |
| dc.contributor.advisor | Milhinhos,Ana | |
| dc.contributor.author | Martins,Diana Cristina da Rocha | |
| dc.contributor.institution | Faculty of Sciences | |
| dc.date.accessioned | 2026-05-19T16:55:02Z | |
| dc.date.available | 2026-05-19T16:55:02Z | |
| dc.date.issued | 2025 | |
| dc.description | Tese de Mestrado, Biologia Molecular e Genética, 2025, Universidade de Lisboa, Faculdade de Ciências | |
| dc.description.abstract | Cork oak (Quercus suber), a Western Mediterranean endemic tree of great ecological and economic importance, is the primary source of cork, a unique and renewable biomaterial whose remarkable protective properties arise primarily from suberin, its major cell wall component. Cork (phellem) is produced outward by the phellogen (cork cambium), which also generates phelloderm inward; together, these tissues constitute the periderm, the outermost protective layer of mature stems and roots. The molecular mechanisms governing suberin deposition and cork formation remain largely unknown. Here, we investigate the role of AINTEGUMENTA (ANT), a transcription factor previously implicated in Arabidopsis thaliana cell proliferation, secondary growth, and suberization. Phylogenetic and expression analyses identified two Q. suber ANT (QsANT) paralogues with complementary expression domains in secondary growth tissues: QsANT LOC112030198 (QsANT-98) is more expressed in xylem, whereas QsANT LOC111985236 (QsANT-36) shows enrichment in periderm tissues. This pattern suggests possible subfunctionalization, with QsANT-98 primarily contributing to vascular-associated processes and QsANT-36 potentially regulating periderm development. To functionally characterize QsANT-98, we employed a genetic complementation approach using A. thaliana as a model system. Arabidopsis ANT loss-of-function mutants (ant-9) revealed defects in periderm proliferation, lateral root initiation, and altered suberization dynamics, while complementation assays showed that QsANT-98 fully restored the ant-9 suberization phenotype but did not rescue periderm or lateral root defects. These findings establish ANT as a regulator at the intersection of secondary growth, barrier formation, and organogenesis, while supporting a model of functional divergence between QsANT paralogues. | en |
| dc.format | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/10400.5/118632 | |
| dc.identifier.url | http://hdl.handle.net/10400.5/118632 | |
| dc.language.iso | eng | |
| dc.subject | AINTEGUMENTA (ANT) | |
| dc.subject | suberization | |
| dc.subject | periderm development | |
| dc.title | Functional characterization of cork oak AINTEGUMENTA in suberization and periderm development via genetic complementation approaches | en |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | embargoedAccess |
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