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Effects of high temperature on mitotic index, microtubule and chromatin organization in rye (Secale cereale L.) root-tip cells

2020Artigo científico Acesso aberto
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dc.contributor.authorNeves, Vanda
dc.contributor.authorViegas, Wanda
dc.contributor.authorCaperta, Ana
dc.date.accessioned2021-09-22T11:23:28Z
dc.date.available2021-09-22T11:23:28Z
dc.date.issued2020
dc.description.abstractStressful high temperatures on plants can limit whole-plant function and decrease crop productivity. However, little is known regarding heat stress effects on microtubule cytoskeleton and chromatin in roots from intact plants. Here we studied high temperature effects on cell division, microtubule and chromatin organization patterns in rye root tips from intact plants subjected to 40ºC for 4 h and after different recovery periods (0RT, 7RT, 24 RT). We showed that heat stress induced changes in nuclear morphology as detected by the unusual presence of interphase cells with irregularly shaped nuclei, probably associated with changes in chromosome segregation at anaphase, leading to micronuclei formation as well as changes in the mitotic index. These alterations were associated to differential effects in microtubules organization in both heat-stressed interphase and mitotic cells at 0RT and 7RT. Although no changes in the distribution of H3 phosphorylation of Ser 10 residues on chromatin were found in cells from heat-stressed plants, marked alterations in chromatin DNA methylation patterns were detected. These effects included higher agglutination of 5-methylcytosine domains in both interphase and metaphase cells compared to controls. Taken together these results seem to suggest that alterations in microtubule conformation upon heat stress influences nuclear chromatin organization and cell cycle progression. However, when seedlings recovered from stress (24RT), root tip cells presented microtubule configurations and chromatin organization patterns similar to controls. We conclude that in spite of heat stress markedly altered cell cycle progression and distribution of epigenetic marks, these responses are transient to cope with such stress conditions in the rootspt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationV. Neves, W. Viegas, A. D. Caperta (2020) Effects of high temperature on mitotic index, microtubule and chromatin organization in rye (Secale cereale L.) root-tip cells. Caryologia 73(4): 55-63pt_PT
dc.identifier.doi10.13128/caryologia-788pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/21911
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFirenze University Presspt_PT
dc.relationPTDC/AGRPRO/4285/2014pt_PT
dc.relationUI/ AGR/04129/2013pt_PT
dc.relationLEAFAGR/ 04129/BPD/2015pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDNA methylationpt_PT
dc.subjectheat stresspt_PT
dc.subjecthistone H3 Ser 10 phosphorylationpt_PT
dc.subjectmicrotubulespt_PT
dc.subjectrootpt_PT
dc.titleEffects of high temperature on mitotic index, microtubule and chromatin organization in rye (Secale cereale L.) root-tip cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleCaryologiapt_PT
person.familyNameViegas
person.familyNameD Caperta
person.givenNameWanda
person.givenNameAna
person.identifier.ciencia-id2A11-692D-B451
person.identifier.ciencia-idD712-FF7D-1A29
person.identifier.orcid0000-0003-4660-1226
person.identifier.orcid0000-0003-0142-8351
person.identifier.scopus-author-id6603353235
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication92899abd-cec8-4145-8907-8cd9d78fc3c4
relation.isAuthorOfPublication0cdf28fa-997b-407d-b8d1-55e836466909
relation.isAuthorOfPublication.latestForDiscovery92899abd-cec8-4145-8907-8cd9d78fc3c4

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