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Effects of high temperature on mitotic index, microtubule and chromatin organization in rye (Secale cereale L.) root-tip cells
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Orientador(es)
Resumo(s)
Stressful high temperatures on plants can limit whole-plant function and
decrease crop productivity. However, little is known regarding heat stress effects on
microtubule cytoskeleton and chromatin in roots from intact plants. Here we studied
high temperature effects on cell division, microtubule and chromatin organization patterns
in rye root tips from intact plants subjected to 40ºC for 4 h and after different
recovery periods (0RT, 7RT, 24 RT). We showed that heat stress induced changes in
nuclear morphology as detected by the unusual presence of interphase cells with irregularly
shaped nuclei, probably associated with changes in chromosome segregation at
anaphase, leading to micronuclei formation as well as changes in the mitotic index.
These alterations were associated to differential effects in microtubules organization in
both heat-stressed interphase and mitotic cells at 0RT and 7RT. Although no changes
in the distribution of H3 phosphorylation of Ser 10 residues on chromatin were found
in cells from heat-stressed plants, marked alterations in chromatin DNA methylation
patterns were detected. These effects included higher agglutination of 5-methylcytosine
domains in both interphase and metaphase cells compared to controls. Taken together
these results seem to suggest that alterations in microtubule conformation upon heat
stress influences nuclear chromatin organization and cell cycle progression. However,
when seedlings recovered from stress (24RT), root tip cells presented microtubule configurations
and chromatin organization patterns similar to controls. We conclude that
in spite of heat stress markedly altered cell cycle progression and distribution of epigenetic
marks, these responses are transient to cope with such stress conditions in the
roots
Descrição
Palavras-chave
DNA methylation heat stress histone H3 Ser 10 phosphorylation microtubules root
Contexto Educativo
Citação
V. Neves, W. Viegas, A. D. Caperta (2020) Effects of high temperature on mitotic index, microtubule and chromatin organization in rye (Secale cereale L.) root-tip cells. Caryologia 73(4): 55-63
Editora
Firenze University Press