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Optimization of MALDI-TOF MSI in grapevine leaves and development of MALDI-TOF MS screening test to evidence if Resveratrol , Pterostilbenes and Synthetic Polysulfane derivates are reversible or irreversible inhibitors of Tubulin
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Resumo(s)
MALDI-TOF MS is a rapid, accurate, specific, and reproducible method that matches all the criteria for the screening ability, it offers great possibilities and potential in terms of reactivity and analysis. The present study was divided in three different subjects: The first one consisted in the optimization of the matrix deposition by a semi- automated matrix spraying by MALDI-TOF MSI for the simultaneous location of Resveratrol, Pterostilbenes and Viniferins on Grapevine leaves. After the optimization of several factors, the optimal parameters are: applied volume= 0.05 µl/ track/ mm of leaf; application speed/ track= 300 nl/s; space between tracks =0,5 mm; energy intensity= 52%. The second one consisted in the development of a MALDI-TOF MS screening test to evidence if Resveratrol and Pterostilbenes are reversible or irreversible inhibitors of Tubulin. And it's possible to conclude that Pterostilbenes are reversible inhibitors and that Resveratrol is or not linked to Tubulin or an irreversible inhibitor. The third and last one concerns to the development of a MALDI-TOF MS screening test to evidence if synthetic polysulfane derivates (DATTS and DBTTS) are reversible or irreversible inhibitors of Tubulin, and it's possible to conclude that both of the compounds are potential reversible inhibitors.
Descrição
Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2015
Palavras-chave
1.4-dybenziltetrasulfane (DBTTS) Diallyltetrasulfide (DATTS) MALDI-TOF MS MSI Matrix Mestrado Integrado - 2015 Pterotstilbenes Resveratrol