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A hipersensibilidade dentinária é uma condição comum, no entanto, esta é frequentemente mal diagnosticada. A abordagem mais adequada é ainda controversa, não havendo literatura esclarecedora em relação a um tratamento definitivo e 100% eficaz. Objectivo: Avaliar, através de um estudo in vitro, os efeitos de 4 dessensibilizantes na superfície dentinária (oclusão dos túbulos dentinários), através da observação ao Microscópio Electrónico de Varrimento (SEM). Materiais e Métodos: Os produtos testados foram: 5% Fluoreto de Sódio (Duraphat®); CPP-ACP em pasta (Tooth Mousse); CPP-ACP em pasta com Flúor (Tooth Mousse plus); 8% de arginina numa base de carbonato de cálcio/ sílica (Colgate Sensitive Pro-Alívio®). Usaram-se para este estudo 8 terceiros molares humanos hígidos, 2 para cada produto. Obteve-se um segmento de coroa de cada dente, com 0,7 mm de câmara pulpar. Cada segmento foi, então, dividido em 5 espécimes, um para cada etapa do protocolo estabelecido (1 Controlo; 2-EDTA; 3 Produto; 4 Saliva Artificial; 5 Solução de Ácido Cítrico 0,02M pH 2,5). Os espécimes foram revestidos por pulverização catódica e observados ao SEM. Resultados: Após análise ao SEM, verificou-se obliteração dos túbulos dentinários nos espécimes submetidos aos produtos dessensibilizantes. No entanto, existem diferenças observáveis entre eles. No espécime FCPP-ACP observa-se uma camada fina e translucida na superfície dos túbulos, contrariamente ao observado no CPP-ACP, onde está presente uma camada espessa formada por cristais de diferentes dimensões. A obliteração pelos produtos Sensitive Pro-Alívio® e Duraphat® é efectuada por deposição de produto no interior dos túbulos. Foram, também, observadas alterações na acção dos produtos após contacto com a Saliva Artificial e solução de Ácido Cítrico. Conclusão: Os quatro dessensibilizantes estudados levam à obliteração dos túbulos dentinários. Essa eficácia poderá ser alterada pela exposição a Saliva Artificial e Ácido Cítrico, sendo que o Duraphat® e a Colgate Sensitive Pro-Alívio® foram os produtos que se apresentaram mais resistentes Dentine hypersensitivity is a common disease, however, is often misdiagnosed. The most suitable approach is still controversial and there are no clear literature about a definitive and 100% effective treatment.
Objective: To evaluate, through an in vitro study, the effect of 4 desensitizing agents on dentin surface (tubule oclusion) by its analysis on the Scanning Electron Microscope (SEM). Materials and Methods: The products tested were: 5% Sodium Fluoride (varnish Duraphat ®), CPP-ACP paste (Tooth Mousse); CPP-ACP paste with fluoride (Tooth Mousse Plus); 8% arginine on the basis of calcium carbonate / silica (Colgate Sensitive Pro-Relief ®). For this study were used 8 human molars, 2 for each product. A segment of each tooth crown was obtained, with 0.7mm pulp chamber. This segment was then divided into 5 samples, one for each step of the established protocol (1-control, 2-EDTA, 3 Product, 4 Artificial Saliva; 5 Solution Citric Acid 0.02 M pH 2.5). The specimens were sputter coated and observed by SEM. Results: After analysis by SEM, it was seen obliteration of the dentinal tubules in the specimens subjected to desensitizing products. However, it is possible to observe differences between them. In the specimen FCPP-ACP there is a thin, translucent layer on the surface of the tubules, in contrast to CPP-ACP, which has a thick layer consisting of crystals of different dimensions. The obliteration by the products Sensitive Pro-Relief ® and Duraphat ® is possible by deposition of product within the tubules. We also observed changes in the action of the products after contact with artificial saliva solution and Citric Acid. Conclusion: The four desensitizing products lead to the obliteration of the dentinal tubules. However, this efficacy is altered by exposure to artificial saliva and citric acid. Duraphat ® and Colgate ® Sensitive Pro-Relief were the products that were more resistant to those alterations.
Objective: To evaluate, through an in vitro study, the effect of 4 desensitizing agents on dentin surface (tubule oclusion) by its analysis on the Scanning Electron Microscope (SEM). Materials and Methods: The products tested were: 5% Sodium Fluoride (varnish Duraphat ®), CPP-ACP paste (Tooth Mousse); CPP-ACP paste with fluoride (Tooth Mousse Plus); 8% arginine on the basis of calcium carbonate / silica (Colgate Sensitive Pro-Relief ®). For this study were used 8 human molars, 2 for each product. A segment of each tooth crown was obtained, with 0.7mm pulp chamber. This segment was then divided into 5 samples, one for each step of the established protocol (1-control, 2-EDTA, 3 Product, 4 Artificial Saliva; 5 Solution Citric Acid 0.02 M pH 2.5). The specimens were sputter coated and observed by SEM. Results: After analysis by SEM, it was seen obliteration of the dentinal tubules in the specimens subjected to desensitizing products. However, it is possible to observe differences between them. In the specimen FCPP-ACP there is a thin, translucent layer on the surface of the tubules, in contrast to CPP-ACP, which has a thick layer consisting of crystals of different dimensions. The obliteration by the products Sensitive Pro-Relief ® and Duraphat ® is possible by deposition of product within the tubules. We also observed changes in the action of the products after contact with artificial saliva solution and Citric Acid. Conclusion: The four desensitizing products lead to the obliteration of the dentinal tubules. However, this efficacy is altered by exposure to artificial saliva and citric acid. Duraphat ® and Colgate ® Sensitive Pro-Relief were the products that were more resistant to those alterations.
Descrição
Tese de mestrado, Medicina Dentária, Universidade de Lisboa, Faculdade de Medicina Dentária, 2013
Palavras-chave
Saúde oral Teses de mestrado - 2013
