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Research Project
Using by-products and non-valuable natural products as flour source to produce gluten free bread - Análise de alimentos super associados a dietas especificas, que não são produzidos em Portugal, para fazer a sua caracterização e comparar com produtos semelhantes, mas de origem nacional.
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Publications
Acorn flour from holm oak (Quercus rotundifolia): Assessment of nutritional, phenolic, and technological profile
Publication . Martins, Rita Beltrão; Gouvinhas, Irene; Nunes, Maria Cristiana; Ferreira, Luís Mendes; Peres, José A.; Raymundo, Anabela; Barros, Ana I.R.N.A.
Acorn is the fruit of holm oak (Quercus rotundifolia), being mainly used nowadays to feed animals, however a
substantial part remains in the fields without any valorization. Underexploited crops are gaining new interest,
driven by food security concerns and health benefits potential as well. In the present work, it was studied the
physicochemical characteristics and functional perspective of acorn flour, as an ingredient for human diet. The
study included nutritional composition analysis, phenolic compounds profile through HPLC, starch content and
its microstructure, fibre, and pasting properties assessment. Acorn flour presented a high content in fat,
particularly monounsaturated and polyunsaturated (oleic and linoleic acids), and high minerals content in
particular K. Concerning phenolic profile, rutin, catechin, ellagic acid, gallic acid, and syringic acid were
identified. In regards to technological profile, fibre was mainly insoluble, with around 11%, and starch content
was 50%. Its pasting behaviour revealed a high gelatinization temperature (85 ◦C), with low breakdown, and
higher retrogradation consistency. These results show acorn flour potential as a valuable and sustainable
multipurpose food ingredient
Food By-Product Valorization by Using Plant-Based Coagulants Combined with AOPs for Agro-Industrial Wastewater Treatment
Publication . Martins, R.B.; Jorge, N.; Lucas, M.S.; Raymundo, Anabela; Barros, A.I.R.N.A.; Peres, J.A.
Re-using and adding value to by-products is one of the current focuses of the agri-food
industry, following the Sustainable Development Goals of United Nations. In this work, the byproducts
of four plants, namely chestnut burr, acorn peel, olive leaf, and grape stem were used as
coagulants to treat elderberry wastewater (EW), a problematic liquid effluent. EW pre-treatment using
these natural coagulants showed promising results after pH and coagulant dosage optimization.
However, the decrease in total organic carbon (TOC) was not significant, due to the addition of
the plant-based natural coagulants which contain carbon content. After this pre-treatment, the
photo-Fenton advanced oxidation process was selected, after preliminary assays, to improve the
global performance of the EW treatment. Photo-Fenton was also optimized for the parameters of
pH, H2O2, Fe2+, and irradiance power, and the best conditions were applied to the EW treatment.
Under the best operational conditions defined in the parametric study, the combined results of
coagulation–flocculation–decantation (CFD) and photo-Fenton for chestnut burr, acorn peel, olive
leaf, and grape stem were, respectively, 90.2, 89.5, 91.5, and 88.7% for TOC removal; 88.7, 82.0, 90.2
and 93.1%, respectively, for turbidity removal; and finally, 40.6, 42.2, 45.3, and 39.1%, respectively, for
TSS removal. As a final remark, it is possible to suggest that plant-based coagulants, combined with
photo-Fenton, can be a promising strategy for EW treatment that simultaneously enables valorization
by adding value back to food by-products
Apple Flour in a Sweet Gluten-Free Bread Formulation: Impact on Nutritional Value, Glycemic Index, Structure and Sensory Profile
Publication . Beltrão Martins, Rita; Nunes, Maria Cristiana; Gouvinhas, Irene; Ferreira, Luís Miguel Mendes; Peres, José Alcides; Barros, Ana Isabel Ramos Novo Amorim; Raymundo, Anabela
Baking bread without gluten presents many challenges generally related with poor sensorial
and nutritional characteristics, and strategies to overcome this issue are needed. Despite many glutenfree
(GF) bread studies, to the best of our knowledge, few are dedicated to sweet GF bread. Sweet
breads have traditionally been an important type of food and are still frequently consumed worldwide.
Apple flour is naturally GF, and is obtained from apples which do not accomplish market quality
requirements and are being wasted. Apple flour was, therefore, characterized in terms of nutritional
profile, bioactive compounds, and antioxidant capacity. The aim of this work was to develop a GF
bread with incorporation of apple flour, in order to study its effect on nutritional, technological, and
sensory characteristics of sweet GF bread. Additionally, in vitro starch hydrolysis and glycemic index
(GI) were also analyzed. Results demonstrated the influence of apple flour in dough’s viscoelastic
behavior, increasing G’ and G”. Regarding bread characteristics, apple flour led to better acceptance
by the consumer, with firmness increasing (21.01; 26.34; 23.88 N), and consequently specific volume
decreasing (1.38; 1.18; 1.13 cm3/g). In addition, an increase of bioactive compounds content and
antioxidant capacity of the breads were revealed. As expected, the starch hydrolysis index increased,
as well as GI. Nevertheless the values were really close to low eGI (56), which is a relevant result for a
sweet bread. Apple flour showed good technological and sensory properties as a sustainable and
healthy food ingredient for GF bread
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Funding agency
Fundação para a Ciência e a Tecnologia
Funding programme
POR_NORTE
Funding Award Number
PD/BD/135332/2017
