A carregar...
Projeto de investigação
Diversity of Lasiodiplodia species associated with grapevine in Portugal and its sustainable control
Financiador
Autores
Publicações
Effect of the Combined Treatments with LC2017 and Trichoderma atroviride Strain I-1237 on Disease Development and Defense Responses in Vines Infected by Lasiodiplodia theobromae
Publication . Reis, Pedro; Mondello, Vincenzo; Diniz, Inês; Alves, Artur; Rego, Cecília; Fontaine, Florence
Grapevine trunk diseases constitute one of the major problems for viticulture worldwide,
with Botryosphaeria dieback considered as one of the most important of these diseases. In this work,
we aimed to (i) evaluate the effect of the combination of two products, Esquive® (a Trichoderma-based
product) and LC2017 (a low-copper-based product), in the control of Lasiodiplodia theobromae, by
evaluating the internal lesion length caused by inoculation of this pathogen on greenhouse kept
grapevines of cvs Cabernet Sauvignon and Touriga Nacional and, (ii) investigating their elicitor
effect on plant defense responses, through the analysis of the expression of a set of genes. The
pathogen was always re-isolated from the infected tissues and was able to cause wood discoloration.
Touriga Nacional exhibited longer lesions than Cabernet Sauvignon, and the application of both
products did not appear to reduce lesion length when compared to LC2017 applied alone. The elicitor
effect of LC2017 on plant defense was confirmed by gene expression analysis, and no significant
differences were found between plants treated with LC2017 and with both products. Moreover, a
specific response related to the cultivar was verified, but this apparently unique interaction between
product, cultivar and pathogen remains to be further investigated
New insights on Botryosphaeria dieback of grapevine: a focus on cultivars response and sustainable control of Lasiodiplodia spp.
Publication . Reis, Pedro Miguel Calhás Jorge; Rego, Maria Cecília Nunes Farinha; Fontaine, Florence; Alves, Artur Jorge da Costa Peixoto
Combining an HA + CU (II) site-targeted copper-based product with a pruning wound protection program to prevent infection with Lasiodiplodia spp. in grapevine
Publication . Reis, Pedro; Gaspar, Ana; Alves, Artur; Fontaine, Florence; Rego, Cecília
The genus Lasiodiplodia has been reported from several grape growing regions and is
considered as one of the fastest wood colonizers, causing Botryosphaeria dieback. The aim of
this study was to (i) evaluate the efficacy of Esquive®, a biocontrol agent, on vineyard pruning
wound protection, applied single or, in a combined protection strategy with a new site-targeted
copper-based treatment (LC2017), and (ii) compare their efficacy with chemical protection provided
by the commercially available product, Tessior®. For two seasons, protectants were applied onto
pruning wounds, while LC2017 was applied throughout the season according to the manufacturer’s
instructions. Pruning wounds of two different cultivars were inoculated with three isolates of
Lasiodiplodia spp. Efficacy of the wound protectants, varied between both years of the assay and
according to the cultivar studied but were able to control the pathogen to some extent. The application
of LC2017 did not show clear evidence of improving the control obtained by the sole application of
the other products tested. Nevertheless, LC2017 showed a fungistatic effect against Lasiodiplodia spp.,
in vitro, and has previously shown an elicitor effect against grapevine trunk diseases. Therefore, this
combination of two protection strategies may constitute a promising long-term approach to mitigate
the impact of Botryosphaeria dieback
First report of dieback caused by Neofusicoccum batangarum in cashew in Guinea-Bissau
Publication . Diniz, Inês; Batista, Dora; Pena, Ana Rita; Rodrigues, Ana Sofia B.; Reis, Pedro; Baldé, Aladje; Indjai, Bucar; Catarino, Luís; Monteiro, Filipa
Early season symptoms of stem, inflorescences and flowers of grapevine associated with Botryosphaeriaceae species
Publication . Reis, Pedro; Gaspar, Ana; Alves, Artur; Fontaine, Florence; Lourenço, Inês; Saramago, José; Mota, Mariana; Rego, Cecília
Botryosphaeria dieback caused by several Botryosphaeriaceae species is one of the most
important grapevine trunk diseases a ecting vineyards worldwide. These fungi cause wedge-shaped
perennial cankers and black streaking of the wood and have also been associated with intervein
leaf chlorosis, dried or mummified berries, and eventually, the death of the plant. Early season
symptoms may sometimes be disregarded by growers, being mistaken with symptoms from other
diseases such as downy mildew or botrytis rot. Currently, few studies are available to determine
what species may be causing these early season symptoms in grapevines. During the 2018 season,
during the flowering period, grapevine samples showing necrosis on green shoots, dried inflorescences,
and flowers, were collected in vineyards throughout the central regions of Portugal. Isolations were
performed from symptomatic organs, and twenty-three isolates of Botryosphaeriaceae were selected.
An analysis of the ITS and part of the translation elongation factor 1- sequences was performed,
revealing that the two main species apparently responsible for these symptoms were Diplodia seriata
and Neofusicoccum parvum. In pathogenicity tests conducted on 1-year-old plants grown under
controlled conditions in a greenhouse and on field-grown clusters, symptoms were reproduced,
confirming the pathogenic behavior of the selection of isolates
Unidades organizacionais
Descrição
Palavras-chave
Contribuidores
Financiadores
Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
Número da atribuição
SFRH/BD/131766/2017