Loading...
6 results
Search Results
Now showing 1 - 6 of 6
- GUIA PRÁTICO DE BIOLOGIA CELULARPublication . Figueiredo, Ana Cristina; Barroso, José Manuel; Pedro, Luís
- HISTOQUÍMICA E CITOQUÍMICA EM PLANTAS: PRINCÍPIOS E PROTOCOLOSPublication . Figueiredo, Ana Cristina; Barroso, José Manuel; Pedro, Luís; Ascensão, Lia
- Chemical Characterization and Bioactivity of Commercial Essential Oils and Hydrolates Obtained from Portuguese Forest Logging and ThinningPublication . Ruas, Ana; Graça, A.; Marto, Joana; Gonçalves, Lídia; Oliveira, Ana; Silva, Alexandra Nogueira da; Pimentel, Madalena; Moura, Artur Mendes; Serra, Ana Teresa; Figueiredo, Ana Cristina; Ribeiro, HelenaEssential oils (EOs) and hydrolates (Hds) are natural sources of biologically active ingredients with broad applications in the cosmetic industry. In this study, nationally produced (mainland Portugal and Azores archipelago) EOs (11) and Hds (7) obtained from forest logging and thinning of Eucalyptus globulus, Pinus pinaster, Pinus pinea and Cryptomeria japonica, were chemically evaluated, and their bioactivity and sensorial properties were assessed. EOs and Hd volatiles (HdVs) were analyzed by GC-FID and GC-MS. 1,8-Cineole was dominant in E. globulus EOs and HdVs, and α- and β-pinene in P. pinaster EOs. Limonene and α-pinene led in P. pinea and C. japonica EOs, respectively. P. pinaster and C. japonica HVs were dominated by α-terpineol and terpinen-4-ol, respectively. The antioxidant activity was determined by DPPH, ORAC and ROS. C. japonica EO showed the highest antioxidant activity, whereas one of the E. globulus EOs showed the lowest. Antimicrobial activity results revealed different levels of efficacy for Eucalyptus and Pinus EOs while C. japonica EO showed no antimicrobial activity against the selected strains. The perception and applicability of emulsions with 0.5% of EOs were evaluated through an in vivo sensory study. C. japonica emulsion, which has a fresh and earthy odour, was chosen as the most pleasant fragrance (60%), followed by P. pinea emulsion (53%). In summary, some of the studied EOs and Hds showed antioxidant and antimicrobial activities and they are possible candidates to address the consumers demand for more sustainable and responsibly sourced ingredients.
- Influence of orchards fertilization management and post-harvest storage time on Malus domestica cv. ‘Gala’ fruit volatiles and quality parametersPublication . Figueiredo, Ana Cristina; da Silva, Anabela Bernardes; Tavares, Cláudia; Pastaneira, Mafalda; Melo, Juliana; Ferro Rodrigues, Carolina; Machado, Alexandra; Antunes, Marília; Cruz, Cristina; da Silva, Jorge Marques; Matos, Ana Rita; Pedro, Luís; Barroso, José; Almeida, Patrícia; Maurício, Anabela; Rosa, Cristina; Faria, João; de Sousa, Miguel L.; Santos, Ricardo; Raimundo, Délio; Oliveira, Cristina“Maçã de Alcobaça” is a type of apple from the West coast of Portugal with Protected Geographical Indication (PGI). This study evaluated the influence of the orchard’s fertilization and post-harvest storage time on the quality of “Maçã de Alcobaça”, ‘Gala’ cultivar. Five orchards were studied for four years, using three fertilization treatments [Standard, twice Standard (2xStandard) and twice Standard including organic matter (2xStandard_OM)]. The fruits’ volatile profile, emanation index, and quality parameters were determined in apples after harvest time (T0), three- (T3) and five months (T5) after storage. At T0, the intact apples volatiles were dominated by trans,trans-α-farnesene (48–79 %). trans,trans-α-Farnesene emanation index was larger in T0 apples independently of the orchard and fertilization treatment, and showed an accentuated decrease from T0 to T5. Fruits quality at T0 and T5, showed significant differences per orchard, but their average weight, TSS, and firmness, were not significantly altered with fertilization treatment. Storage did not significantly affect the fruits weight, but TSS and firmness always decreased regardless of the orchard or fertilization treatment. Among other things, the observed variations among orchards after fertilization treatments may relate to the cultural practices of each orchard as well as the fact that their ages and rootstocks varied.
- Potencialidades e Aplicações das Plantas Aromáticas e Medicinais. Curso Teórico-PráticoPublication . Figueiredo, Ana Cristina; Barroso, José Manuel; Pedro, LuísAs plantas aromáticas e medicinais fazem parte do nosso dia-a-dia, como alimento, mézinha, sob a forma de cosmético e/ou pelo seu aroma. Constituindo um recurso natural de valor ímpar, urge conhecê-las, saber valorizá-las e utilizá-las de uma forma sustentada. Em Portugal, como no resto do Mundo, tem-se assistido, nos últimos anos, a um ressurgir da vontade de consumir o que é natural. Contudo, muito daquele que era um inestimável saber e saber-fazer popular sobre plantas e produtos naturais foi-se, simultaneamente, perdendo, num período de tempo em que pouco se valorizou este conhecimento e em que a forma de vida ao tornar-se mais citadina, perdeu muito destes valores. Ainda assim, ao longo de vários anos, muitas Instituições Nacionais foram desenvolvendo inúmeros trabalhos sobre plantas aromáticas e medicinais, acumulando uma mais-valia preciosa de conhecimento em várias vertentes deste domínio. Este Curso nasceu da vontade de divulgar à comunidade em geral, o muito do que tem sido feito em muitas destas Instituições sobre esta temática. Conscientes de que os assuntos a abordar não se esgotam aqui e que muito fica ainda por abranger, esperamos que este Curso seja oportuno e útil e constitua um valioso acervo de informação para todos quantos se interessam, ou convivem diariamente, directa ou indirectamente, com plantas aromáticas e medicinais. A Comissão Organizadora (Nota Prévia da 1ª e 2ª Edição)
- Azorean Cryptomeria japonica immature female cones essential oil: Effect of hydrodistillation fractionation on the chemical composition and in vitro antifungal activity against Thielaviopsis paradoxaPublication . Arruda, Filipe; Lima, Ana; Janeiro, Alexandre; Rodrigues, Tânia; Baptista, José; Rosa, José S.; Machado, Alexandra; Figueiredo, Ana Cristina; Lima, ElisabeteCryptomeria japonica's wood production in Azores generates large amounts of underutilized biomass residues, such as immature female cones (Az–CJIFC), that can be used to produce essential oils (EOs). Hydrodistillation (HD) can be used both to obtain and to fractionate EOs. In this study, EOs from Az–CJIFC, grinded (GR) and non-grinded (NGr) fresh samples, were obtained via HD over 4 h, yielding 1.0 % and 0.5 %, w/w, respectively. Thus, GR Az–CJIFC was chosen to obtain six EO fractions (Frs. 1–6), collected at sequential HD timeframes (HDTs: 0–2, 2–10, 10–30, 30–60, 60–120, and 120–240 min). The obtained EO samples (crude EOs and fractions) were evaluated for their chemical composition (GC–FID/GC–MS analyses) and antifungal activity (micro-atmosphere method) against phytopathogenic fungi (Penicillium italicum, P. digitatum and Thielaviopsis paradoxa). Results indicated that all samples were active only towards T. paradoxa, however, with a differential efficacy, due to their specific composition. Fraction 4 vapor treatment displayed the strongest activity, but lower than that of (–)-terpinen-4-ol, a key oxygen-containing monoterpene (OCM) of Az–CJIFC EO, peaking its concentration in Fr3 (14.5 %) and Fr4 (13.8 %). This latter fraction was the richest in the OCM α-terpineol (a minor Az–CJIFC EOs' component). On the other hand, Frs.1–3 and EOs were dominated by monoterpene hydrocarbons (65.0–96.5 %), mainly α-pinene (19.0–28.4 %) and sabinene (19.9–50.5 %), while Frs.5 and 6 were the richest in oxygen-containing sesquiterpenes (47.1–70.8 %; chiefly elemol plus α-, β- and γ-eudesmol) and diterpene hydrocarbons (5.2–6.4 %; mostly phyllocladene). In conclusion, new high value-added products can now be targeted in Az–CJIFC EO by adjusting the HDT, with potential importance in pineapple fruit black rot disease management caused by T. paradoxa on Ananas comosus in the Azores, and also contributing for the local C. japonica’s EO industry development and sustainable circular bio-economy.