Browsing by Issue Date, starting with "2021-08-05"
Now showing 1 - 5 of 5
Results Per Page
Sort Options
- A single-step multicomponent synthesis of a quinoline derivative and the characterization of its cyclodextrin inclusion complexPublication . Pinto, Luciana Matos Alves; Adeoye, Oluwatomide; Thomasi, Sérgio Scherrer; Francisco, Ana Paula; Marques, Helena CabralMulticomponent synthesis are convergent reactions in which three or more starting materials react in a single step to form the product. In the discovery and development of new drugs, multicomponent reactions offer several advantages over traditional synthesis: shorter experiment time, less need for laboratory techniques and scalability. Thus, the objective of this work was to perform the multicomponent synthesis of a hexahydroquinoline compound whose pharmacological activity has been described previously. The synthesis product was characterized by spectroscopic techniques such as Fourier transform infrared spectrometry and 1H, 13C and HMQC nuclear magnetic resonance (NMR). The mechanism of the reaction was proposed to include a conjugated Michael addition. Subsequently, inclusion complexes (ICs) at 1:1 and 1:2 stoichiometries were prepared with the appropriate cyclodextrin to increase the aqueous solubility and bioavailability of this molecule. Kneading and lyophilization (LYO) methods were used to prepare the inclusion complexes, which were evaluated by phase solubility and 1H NMR spectroscopy, with LYO at a 1:2 stoichiometry having the best results. The ICs were then characterized by differential scanning calorimetry, near-infrared spectroscopy and in vitro dissolution tests, which verified a slower release of the compound with the potential to improve its biological properties.
- Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalencePublication . Grilo, Miguel; Chambel, Lélia; Marques, Tiago A.; Sousa-Santos, Carla; Robalo, Joana; Oliveira, ManuelaAssessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity and load, as well as in the prevalence and proportion of skin lesions, in fishes exposed to similar environmental conditions, an observational study was implemented. Using a set of 12 individuals belonging to two sympatric Iberian leuciscid species (Squalius pyrenaicus and Iberochondrostoma lusitanicum), the skin lesion score in each individual was analyzed. Furthermore, a bacterial collection of Aeromonas spp. isolated from each individual was created and isolates’ load was quantified by plate counting, identified at species level using a multiplex-PCR assay and virulence profiles established using classical phenotypic methods. The similarity relationships of the isolates were evaluated using a RAPD analysis. The skin lesion score was significantly higher in S. pyrenaicus, while the Aeromonas spp. load did not differ between species. When analyzing Aeromonas species diversity between fishes, different patterns were observed. A predominance of A. hydrophila was detected in S. pyrenaicus individuals, while I. lusitanicum individuals displayed a more diverse structure. Similarly, the virulence index of isolates from S. pyrenaicus was higher, mostly due to the isolated Aeromonas species. Genomic typing clustered the isolates mainly by fish species and skin lesion score. Specific Aeromonas clusters were associated with higher virulence indexes. Current results suggest potential differences in susceptibility to Aeromonas spp. at the fish species/individual level, and constitute important knowledge for proper wildlife management through the signalization of at-risk fish populations and hierarchization of conservation measures.
- Usos e perceções sobre jardins e parques públicos urbanos. Resultados preliminares de um inquérito na cidade do Porto (Portugal)Publication . Guedes Vidal, Diogo; Fernandes, Cláudia; Viterbo, Lilian Monteiro Ferrari; Vilaça, Helena; Barros, Nelson; Maia, RuiA crescente concentração populacional nas cidades é reconhecida como um dos principais desafios contemporâneos. É nas cidades que os desafios sociais e ambientais se agigantam, implicando um olhar sobre o planeamento urbano sustentável. Os espaços verdes urbanos desempenham um papel importante na resposta a tais desafios, concretamente através dos serviços dos ecossistemas. Os serviços culturais ganham especial relevo, nomeadamente na melhoria da saúde mental, física e social dos seus utilizadores. Este trabalho tem como objetivos conhecer os usos e as perceções dos utilizadores dos jardins e parques públicos da cidade do Porto, bem como de que forma esses mesmos espaços estão a satisfazer as suas necessidades, estabelecendo uma relação com a localização dos mesmos na cidade tendo em conta uma maior ou menor privação socioeconómica e ambiental. Partindo de inquérito por questionário em fase de aplicação aos utilizadores de 25 jardins e parques de acesso público, propõem-se uma discussão sobre os resultados preliminares obtidos (n=131). Os resultados permitem um esboçar do perfil dos potenciais utilizadores destes espaços, além de revelarem que espaços da cidade em áreas de maior privação socioeconómica e ambiental são percecionados pelos seus utilizadores como espaços verdes com menor qualidade. Espera-se que os resultados auxiliem na definição de estratégias de atratividade dos espaços verdes da cidade que possuem menor qualidade e se inserem em zonas de maior privação.
- Exercícios de EconometriaPublication . Lopes, Artur SIlva
- Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions' prevalencePublication . Grilo, Miguel; Chambel, Lélia; Marques, Tiago A.; Sousa-Santos, C.; Robalo, Joana; Oliveira, ManuelaAssessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity and load, as well as in the prevalence and proportion of skin lesions, in fishes exposed to similar environmental conditions, an observational study was implemented. Using a set of 12 individuals belonging to two sympatric Iberian leuciscid species (Squalius pyrenaicus and Iberochondrostoma lusitanicum), the skin lesion score in each individual was analyzed. Furthermore, a bacterial collection of Aeromonas spp. isolated from each individual was created and isolates’ load was quantified by plate counting, identified at species level using a multiplex-PCR assay and virulence profiles established using classical phenotypic methods. The similarity relationships of the isolates were evaluated using a RAPD analysis. The skin lesion score was significantly higher in S. pyrenaicus, while the Aeromonas spp. load did not differ between species. When analyzing Aeromonas species diversity between fishes, different patterns were observed. A predominance of A. hydrophila was detected in S. pyrenaicus individuals, while I. lusitanicum individuals displayed a more diverse structure. Similarly, the virulence index of isolates from S. pyrenaicus was higher, mostly due to the isolated Aeromonas species. Genomic typing clustered the isolates mainly by fish species and skin lesion score. Specific Aeromonas clusters were associated with higher virulence indexes. Current results suggest potential differences in susceptibility to Aeromonas spp. at the fish species/individual level, and constitute important knowledge for proper wildlife management through the signalization of at-risk fish populations and hierarchization of conservation measures.