Browsing by Author "Struys, EA"
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- Increased, homocysteine and S-adenosylhomocysteine concentrations and DNA hypomethylation in vascular diseasePublication . Castro, R; Rivera, I; Struys, EA; Jansen, EEW; Ravasco, P; Camilo, ME; Blom, HJ; Jakobs, C; de Almeida, ITBackground: The pathogenic mechanism of homocysteine's effect on cardiovascular risk is poorly understood. Recent studies show that DNA hypomethylation induced by increases in S-adenosylhomocysteine (AdoHcy), an intermediate of Hcy metabolism and a potent
- Intracellular S-adenosylhomocysteine increased levels are associated with DNA hypomethylation in HUVECPublication . Castro, R; Rivera, I; Martins, C; Struys, EA; Jansen, EEW; Clode, N; Graca, LM; Blom, HJ; Jakobs, C; de Almeida, ITHyperhomocysteinemia is a risk factor for atherosclerosis and vascular disease; however, the mechanism underlying this association remains poorly understood. Increased levels of intracellular S-adenosylhomocysteine (AdoHcy), secondary to homocysteine-medi
- Quantification of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent 1,N-6-etheno derivativesPublication . Castro, R; Struys, EA; Jansen, EEW; Blom, HJ; de Almeida, IT; Jakobs, CA simplified reversed phase HPLC system for the detection of fluorescent 1,N-6-etheno derivatives of SAM (S-adenosylmethionine) and S-adenosylhomocysteine (SAH) is described. The most important changes from the previously reported method are a shorter der
- Quantitative analysis of urinary acylglycines for the diagnosis of beta-oxidation defects using GC-NCI-MSPublication . Costa, CG; Guerand, WS; Struys, EA; Holwerda, U; ten Brink, HJ; de Almeida, IT; Duran, M; Jakobs, CThe analysis of acylglycines is an important biochemical tool for the diagnosis of inherited disorders of mitochondrial fatty acid P-oxidation. A stable isotope dilution gas chromatography negative chemical ionisation mass spectrometry method for the quantitative analysis of short and medium-chain acylglycines as their bis(trifluoromethyl)benzyl (BTFMB) ester derivatives is described. The diagnostic usefulness of the method was demonstrated in nine patients with medium-chain acyl-coenzyme A (CoA) dehydrogenase (MCAD) deficiency, and seven patients with multiple acyl-CoA dehydrogenation defect (MAD). The urinary acylglycine profiles in these patients were compared to those in controls (n = 19), children on a medium-chain triglyceride (MCT) supplemented diet (n = 4), and patients with various other diseases (n = 5). (C) 2000 Elsevier Science B.V. All rights reserved.