A carregar...
Publicação
Estudos de propagação in vitro, caracterização e valorização de carqueja (Pterospartum tridentatum (L.) Willk)
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 7.72 MB | Adobe PDF |
Resumo(s)
The aim of this work was to develop methodologies for micropropagation and extraction of
bioactive compounds from both wild gorse (Pterospartum tridentatum L.) and
micropropagated gorse explants, as a means of valorisation of this species.
Micropropagation was very effective on large scale propagation, having multiplication rates
ranging from 1.6 to 43.9; number of shoots ranging from 1.5 to 10.4 and rooting above
96.7%.
The extraction yield of aqueous extraction varied from 10.5 to 19.4 and 12.2 to 30.1% (w/w)
for wild gorse and micropropagated gorse explants, respectively. Aqueous extracts showed
high levels of total phenols, from 203.4 to 369.2 and 104.1 to 121.3 mg gallic acid eq g-1 ms,
and FRAP antioxidant activity: 256-856 and 160-260 mmolFe2+ 100g-1 ms; DPPH: 1433-1551
and 1503-1509 mM Trolox 100g-1 ms for wild gorse and micropropagated gorse explants,
respectively.
Extracts showed no antifungal activity. However, these extracts showed inhibition of Bacillus
subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Chitosan bioactive coatings
with gorse extracts improved cherry postharvest preservation ability. The results allow
topropose these methodologies to obtain extracts with bioactive properties from
micropropagated material, without depleting natural resources
Descrição
Doutoramento em Engenharia Alimentar - Instituto Superior de Agronomia
Palavras-chave
Pterospartum tridentatum ethnobotany micropropagation aqueous extracts biological activity
Contexto Educativo
Citação
Coelho, M.T.P.P.G.R. - Estudos de propagação in vitro, caracterização e valorização de carqueja (Pterospartum tridentatum (L.) Willk). Lisboa: ISA, 2015, 287 p.