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Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes

2019Journal article Open access
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dc.contributor.authorSkalska, Julia
dc.contributor.authorOliveira, Filipa
dc.contributor.authorFigueira, Tiago N.
dc.contributor.authorMello, Érica O.
dc.contributor.authorGomes, Valdirene M.
dc.contributor.authorMcNaughton-Smith, Grant
dc.contributor.authorCastanho, Miguel A. R. B.
dc.contributor.authorGaspar, Diana
dc.date.accessioned2023-04-21T11:41:24Z
dc.date.available2023-04-21T11:41:24Z
dc.date.issued2019
dc.descriptionThis journal is © The Royal Society of Chemistry 2019pt_PT
dc.description.abstractOne of the most important causes of failure in tumour treatment is the development of resistance to therapy. Cancer cells can develop the ability to lose sensitivity to anti-neoplastic drugs during reciprocal crosstalk between cells and their interaction with the tumour microenvironment (TME). Cell-to-cell communication regulates a cascade of interdependent events essential for disease development and progression and can be mediated by several signalling pathways. Exosome-mediated communication is one of the pathways regulating these events. Tumour-derived exosomes (TDE) are believed to have the ability to modulate TMEs and participate in multidrug resistance mechanisms. In this work, we studied the effect of the natural defensin from common bean, PvD1, on the formation of exosomes by breast cancer MCF-7 cells, mainly the modulatory effect it has on the level of CD63 and CD9 tetraspanins. Moreover, we followed the interaction of PvD1 with biological and model membranes of selected composition, by biophysical and imaging techniques. Overall, the results show that PvD1 induces a dual effect on MCF-7 derived exosomes: the peptide attenuates the recruitment of CD63 and CD9 to exosomes intracellularly and binds to the mature exosomes in the extracellular environment. This work uncovers the exosomemediated anticancer action of PvD1, a potential nutraceutical agent.pt_PT
dc.description.sponsorshipThe authors thank Fundação para a Ciência e a Tecnologia (FCT I.P., Portugal) for funding – PTDC/BBB-BQB/1693/2014, and also acknowledge financial support from the Brazilian agencies CNPq, CAPES, and FAPERJ (E-26/203.090/2016; E-26/202.132/2015). Julia Skalska, Filipa D. Oliveira, Tiago N. Figueira and Diana Gaspar acknowledge FCT I.P. for fellowships PD/BD/114177/2016, PD/BD/135046/2017, SFRH/BD/5283/2013 and SFRH/BPD/109010/2015 respectively. Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) is also acknowledged for funding: call H2020-MSCA-RISE-2014, Grant agreement 644167, 2015–2019.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanoscale, 2019, 11, 23366–23381pt_PT
dc.identifier.doi10.1039/C9NR07843Fpt_PT
dc.identifier.eissn2040-3372
dc.identifier.issn2040-3364
dc.identifier.urihttp://hdl.handle.net/10451/57218
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationPD/BD/114177/2016pt_PT
dc.relationCell-cell communication in metastatic breast cancer: a control point that can lead to breast cancer treatment
dc.relationSFRH/BD/5283/2013pt_PT
dc.relationCell-cell direct/indirect communication in metastatic breast cancer as a control point in oncotherapy
dc.relation.publisherversionhttps://pubs.rsc.org/en/journals/journalissues/nr#!recentarticles&advpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titlePlant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCell-cell communication in metastatic breast cancer: a control point that can lead to breast cancer treatment
oaire.awardTitleCell-cell direct/indirect communication in metastatic breast cancer as a control point in oncotherapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BQB%2F1693%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F109010%2F2015/PT
oaire.citation.endPage23381pt_PT
oaire.citation.issue48pt_PT
oaire.citation.startPage23366pt_PT
oaire.citation.titleNanoscalept_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameOliveira
person.familyNameFigueira
person.familyNameCastanho
person.familyNameGaspar
person.givenNameFilipa
person.givenNameTiago
person.givenNameMiguel
person.givenNameDiana
person.identifier103438
person.identifier.orcid0000-0001-8816-9932
person.identifier.orcid0000-0002-0813-0745
person.identifier.orcid0000-0001-7891-7562
person.identifier.orcid0000-0002-9602-567X
person.identifier.ridM-9562-2015
person.identifier.scopus-author-id56605575600
person.identifier.scopus-author-id55180303000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery3888b418-93b3-4355-85e5-16c4042e3b48
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