dc.contributor.advisor	Duclos, Joana
dc.contributor.advisor	Kan, Jan van
dc.contributor.author	Duarte, José Manuel Martins Abrantes Teixeira
dc.date.accessioned	2009-02-18T14:57:27Z
dc.date.available	2009-02-18T14:57:27Z
dc.date.issued	2008
dc.description	Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia	en
dc.description.abstract	Botrytis cinerea [teleomorph: Botryotinia fuckeliana] is a heterothallic filamentous plant pathogenic Ascomycete with over two hundred different hosts, and its control at the crop level is still very difficult to achieve. Sexual reproduction as an active part in the fungus dispersal and variability has been underestimated until recent years. Research leading to more insight into the MAT locus and the fundamental principles of the mating process can prompt new strategies for B. cinerea control. During the B. cinerea infection process, phytotoxic proteins such as NEP1 are produced. Their function is yet to be unraveled, but it was postulated that they might play a role in speciation in the genus Botrytis. This report describes a mating type screening of a population of 80 field isolates of B. cinerea. Findings indicate a typical 1:1 Mendelian distribution of the MAT loci, and one strain with both MAT genes is reported. A scan of the MAT locus suggests the absence of the MAT1-1 locus in dual mating strains. Furthermore, Southern blotting demonstrates for the first time the possible presence of a complete MAT1-1 locus in a dual mating strain, instead of the predicted incomplete MAT1-1 locus (dMAT1). Besides the MAT loci, genomic sequences of the BcNEP1 gene in 16 different B. cinerea strains are analyzed, and results demonstrate the existence of three haplotypes and two alleles. Purifying selection is accounted for evolutionary changes within the gene.Finally, four uORF independent knockout mutants were created. This predicted gene is located within the MAT1-1 locus, but its function is yet to be described.	en
dc.identifier.uri	http://hdl.handle.net/10400.5/540
dc.language.iso	eng	en
dc.subject	Botrytis cinerea	en
dc.subject	genes MAT	en
dc.subject	proteinas NEP	en
dc.subject	silenciamento genético	en
dc.subject	MAT genes	en
dc.subject	NEP proteins	en
dc.subject	gene knockout	en
dc.title	Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis	en
dc.type	master thesis
dspace.entity.type	Publication
rcaap.rights	openAccess	en
rcaap.type	masterThesis	en

Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis

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